NeST Long ncRNA 调控IFN-gama.pdf

The NeST Long ncRNA Controls Microbial Susceptibility and Epigenetic Activation of the Interferon-g Locus J. Antonio Gomez,1 Orly L. Wapinski,2 Yul W. Yang,2 Jean-Franc¸ ois Bureau,3 Smita Gopinath,1 Denise M. Monack,1 Howard Y. Chang,2 Michel Brahic,1 and Karla Kirkegaard1,* 1Department of Microbiology and Immunology 2Program in Epithelial Biology, Howard Hughes Medical Institute Stanford University School of Medicine, Stanford, CA 94305, USA 3 ´ Departement de Virologie, Institut Pasteur, 75724 Paris Cedex 15, France *Correspondence: karlak@ /10.1016/j.cell.2013.01.015 SUMMARY mammalian susceptibility to infection or the immune response to pathogens has not been previously described. Long noncoding RNAs (lncRNAs) are increasingly NeST , formally known as Tmevpg1, is an lncRNA gene located appreciated as regulators of cell-specific gene adjacent to the interferon (IFN)-g-encoding gene in both mice expression. Here, an enhancer-like lncRNA termed (Ifng) and humans (IFNG). NeST was originally identified as a NeST (nettoie Salmonella pas Theiler’s [cleanup candidate gene in a susceptibility locus for Theiler’s virus Salmonella not Theiler’s]) is shown to be causal (NeST stands for nettoie Salmonella pas Theiler’s [cleanup Salmonella not Theiler’s]). In both mouse and human genomes, for all phenotypes conferred by murine viral sus- NeST RNA is encoded on the DNA strand opposite to that coding ceptibility locus Tmevp3. This locus was defined