瘀血阻并滞证糖尿病足大鼠模型的建立.pdfVIP

第 27 卷 第 2期 Vol. 27 No. 2 2 0 0 9 年 2 月 CH INESE ARC H IV ES OF T RAD IT IONAL C H INES E M ED IC INE Feb. 2 0 0 9 陶茂灿, 曹 毅, 许经纶, 黄 平 (, 310006) : 目的: 建立瘀血阻滞证糖尿病足大鼠动 模型方法: 给 SD雄性大鼠腹腔注射链脲佐菌素后, 降低 环境温度, 造成糖尿病足动 模型; 同时监测各组大鼠的体重饮水量空腹血糖和血液流变学指标, 观察各组大 鼠舌下耳廓脉络变化, 进行各组大鼠肢端坏疽症状评分结果: 糖尿病足造模成功率 75% 糖尿病足组与正常 对照不降温组降温组相比, 体重明显减轻, 饮水量明显增加, 空腹血糖肢端坏疽症状评分血液流变学全血黏度 低切中切高切红细胞聚集指数均明显升高, 舌质紫黯, 舌下脉络增长增粗, 耳廓苍白或紫黯结论: 本方法可 成功建立瘀血阻滞证糖尿病足大鼠模型, 为探讨糖尿病足的发病机理和筛选药 奠定基础 :糖尿病足; 瘀血阻滞证; 动 模型; 大鼠 : R- 332 : A : 1673- 77 17( 2009) 02- 0353- 03 T he E stab lishm ent of D iabetic Foot RatM ode l w ith B lood S tasis Syndrom e TAO M ao an, CAO Y ,i XU Jinglun, HUANG P ing ( Zhej iang Ch inese M edi a lH osp ital, H angzhou 310006, Zhejiang, Ch ina) A b stract: O bj ect iv e: T o estab lish a b lood stasis syndrom e of d iabeti foot rat m odelM ethod s: SD m ale rats w ere in je ted STZ, lower the tem perature, the anmi al models of diabeti foot w ere established. A t the same tmi e m onitoring the weight of the rats, w ater quantity, fasting blood sugar and b lood rheology ind i ators, observing the rats sublingua,l auri le vessel hanges, s oring the rats a rom eli gangrene symptom.R esults: The diabeti foot m odel su ess rate of 75 per en t. Compared w ith not redu ed tem perature group, redu ed temperature group, wh i h both are norm al ontrol groups, the rats in the diabeti foot group sign ifi antly lose body w eight, drunk more, and w ith high fasting bloodglu ose, h ighs ore of a rom eli gangrene sym ptom. The h igh, m idd le and low shear of blood vis osity, the RBC a umu lation index of rats in the diabeti foot group in reased greatly. T ongue body is dark purp le, sub lingual vesse