辅助生殖技术子代印迹基因IGF2与H19mRNA表达的初步研究.docVIP

辅助生殖技术子代印迹基因IGF2与H19mRNA表达的初步研究 摘要：目的：通过研究辅助生殖技术（ART）子代印迹基因IGF2mRNA与H19的表达水平及其与出生情况的关系，初步评估ART操作对子代的生长发育情况及出生缺陷的影响，以探索ART操作的安全性 。方法：以58例ART子代（40例异卵双胎与18例单胎）作为研究组，同期32例自然妊娠子代（30例单胎与2例异卵双胎）作为对照组，记录母龄、分娩孕周、出生体重及有无出生缺陷等，收集新生儿脐带血，使用real-time PCR技术检测其印迹基因IGF2mRNA与H19的表达水平，使用SPSS统计软件进行分析。结果：所有纳入研究的新生儿均未发现有出生缺陷。研究组与对照组比较，印迹基因IGF2mRNA的表达有显著性差异（p0.05），主要表现为ART双胎与自然妊娠单胎比较有显著性差异（p0.05），单胎之间比较差异无显著性（p0.05）； 研究组与对照组H19表达有显著性差异（p0.05），但ART双胎/单胎与自然妊娠单胎比较H19表达无显著性差异。出生体重与H19 表达水平有显著负相关关系（p0.05）；分娩周数与IGF2表达水平有显著负相关关系（p0.05）；印记基因IGF2与H19表达水平有显著正相关关系（p0.05）。结论：ART双胎妊娠率较高，继而引起早产并低体重儿发生率较高，印迹基因表达水平的改变亦可能与此有关，但并未增加出生缺陷的风险，对于ART操作的远期安全性需要做更进一步的研究。 关键词：辅助生殖技术;印迹基因;IGF2;H19 Expression levels of imprinted genes IGF2 and H19 of the offspring conceived by assisted reproductive technology Ni Yun-ping, Chen Shi-ling, Zhong Mei, Zheng Hai-yan, Wu Fang-rong,Shi Xiao-yun,Luo Yan-qun,Xia Rong Center of Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Corresponding author: Chen Shi-ling, Center of Reproductive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515，China . E-mail：chensl_92@163.com’ cord-blood, testing their expression levels of imprinted genes IGF2 and H19 by real- time PCR. Results: None of the cases collected were born with birth defects, even the cases showed variability of IGF2/H19 gene expression.There existed significant difference between ART group and natural conceived group with the expression levels of IGF2（p0.05）;the difference of the expression levels of H19 was also significantly（p0.05）.The correlation between birth weight and expression levels of H19 gene was significantly（p0.05）, so was gestational age and expression levels of IGF2 gene（p0.05）.The correlation of expression levels between the two imprinted genes was significantly too（p0.05）.Conclusion: High twin pregnancy rate of ART is likely to result in preterm birth and low birth weight, which might has some conne