两株内生真菌对菊花抗盐特性的影响-天津中草药杂志社.PDF

• 158 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 1 期 2011 年 1 月 两株内生真菌对菊花抗盐特性的影响 1 1 2 1* 刘晓珍 ，宋文玲 ，蔡信之 ，戴传超 1. 南京师范大学生命科学学院，江苏 南京 210046 2. 盐城师范学院，江苏省滩涂生物资源与环境保护重点实验室，江苏 盐城 224002 摘 要：目的 用 NaCl 模拟盐胁迫，研究接种内生真菌对菊花抗盐特性的影响。方法 采用盆栽试验，以与内生真菌葡萄 孢（C1 菌株）、球毛壳菌（C4 菌株）共生培养的菊花为材料，研究不同浓度盐胁迫对不同处理组菊花生理指标的影响。结 果 各处理组菊花根叶的含水量随着盐分胁迫的加重而降低，接菌处理组的根叶含水量减失程度比对照组小。各处理组 SOD 酶活力、可溶性蛋白量均随 NaCl 浓度提高而增加，20 g/L NaCl 时均达到最大值，接菌处理组 SOD 酶活力、可溶性蛋白量 高于对照组，C4 组高于 C1 组。POD 酶活力均先升高后降低，15 g/L NaCl 时各处理组POD 酶活力达到最大值，C4、C1 组 POD 酶活力分别比对照组高 25.50% 、1.35%。15 g/L NaCl 处理时，C4 组 PAL 活力约是对照组的 7 倍。结论 内生真菌增 加了菊花的抗盐能力，C4 组效果好于 C1 组。 关键词：内生真菌；菊花；盐胁迫；抗盐特性；酶；葡萄孢；球毛壳菌 中图分类号：R282.1 文献标志码：A 文章编号：0253 - 2670(2011)01 - 0158 - 06 Effect of two kinds of endophytic fungi on salt resistance of Chrysanthemum morifolium 1 1 2 1 LIU Xiao-zhen , SONG Wen-ling , CAI Xin-zhi , DAI Chuan-chao 1. College of Life Science, Nanjing Normal University, Nanjing 210046, China 2. Jiangsu Provincial Key Laboratory of Low Beach Biology Resources Environment Protection, Yancheng Normal College, Yancheng 224002, China Abstract: Objective The NaCl stress conditions were simulated to study the effect of the endophytic fungi C1, C4 on antisalty characteristic of Chrysanthemum morifolium in the adverse circumstance. Methods Endophytic Botrytis sp. (C1) and Chaetomium globosum (C4) were inoculated to the C. morifolium plantlets which were planted in the pots in order to research the effects of salt stress on physiological indicators of C. morifolium. Results With the increase of NaCl concentration, the water content of root and leaf decreased in every group. The loss of root and leaf′s water in fungi-treated group was smaller than that in the control group. SOD activities in every group increased with