单个b细胞抗体制备技术及应用-微生物学报.pdfVIP

迟象阳 等/单个B 细胞抗体制备技术及应用 651 生 物 工 程 学 报 Chinese Journal of Biotechnology June 25, 2012, 28(6): 651−660 /cjbcn ©2012 Chin J Biotech, All rights reserved 特邀综述 B 迟象阳，于长明，陈薇 军事医学科学院生物工程研究所，北京 100071 迟象阳, 于长明, 陈薇. 单个B 细胞抗体制备技术及应用. 生物工程学报, 2012, 28(6): 651−660. Chi XY, Yu CM, Chen W. Single B cell monoclonal antibody technologies and applications. Chin J Biotech, 2012, 28(6): 651−660. 摘 要: 单克隆抗体是现代生命科学研究的重要工具，为许多领域的发展作出了不可估量的贡献。随着 PCR 技术和单克隆抗体技术的发展和成熟，单个B 细胞抗体制备技术迅速兴起。该技术能够对单个的抗原特异性 B 细胞进行抗体基因的体外克隆和表达，保证了轻重链可变区的天然配对，相较于传统的抗体制备技术具 有效率高、全人源、基因多样性更丰富等优势。单个B 细胞抗体制备技术已成为制备全人抗体的热门方法， 同时也促进了包括抗体发生成熟、疫苗保护机制、疫苗开发、肿瘤及自身免疫疾病等免疫学相关研究。文中就 单个B 细胞抗体制备技术的过程及应用作简要综述。 关键词: 单克隆抗体，单个 B 细胞，免疫球蛋白基因，逆转录PCR Single B cell monoclonal antibody technologies and applications Xiangyang Chi, Changming Yu, and Wei Chen Beijing Institute of Biotechnology, Beijing 100071, China Abstract: Monoclonal antibodies (mAbs) contribute a lot to the development of numerous fields in life science as a pivotal tool in modern biological research. Development of the PCR methods and maturation of antibody production have made it possible to generate mAbs from single human B cells by single cell RT-PCR with successional cloning and expression in vitro. Compared to traditional monoclonal antibody technologies, single B cell technologies require relatively fewer cells, which are highly efficient in obtaining specific mAbs in a rapid way with preservation of the natural heavy and Received: November 30, 2011; Accepted: February 14, 2012 Supported by: National Natural Science Foundation of China (No., National Science Fund for Distinguished Yo