MR医学影像文献Yang-2016-A combination strategy for extractio.pdfVIP

Journal of Chromatography A, 1431 (2016) 184–196 Contents lists available at ScienceDirect Journal of Chromatography A j o u r n a l h o me p a g e : w w w. elsev /locate/chro ma A combination strategy for extraction and isolation of multi-component natural products by systematic two-phase solvent extraction-13C nuclear magnetic resonance pattern recognition and following conical counter-current chromatography separation: Podophyllotoxins and flavonoids from Dysosma versipellis (Hance) as examples Zhi Yang, Youqian Wu, Shihua Wu ∗ Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China a r t i c l e i n f o a b s t r a c t Article history: Despite of substantial developments of extraction and separation techniques, isolation of natural products Received 28 July 2015 from natural resources is still a challenging task. In this work, an efficient strategy for extraction and isola- Received in revised form tion of multi-component natural products has been successfully developed by combination of systematic 23 December 2015 13 two-phase liquid–liquid extraction- C NMR pattern recognition and following conical counter-current Accepted 28 December 2015 Available online 31 December 2015 chromatography separation. A small-scale crude sample was first distributed into 9 systematic hexane- ethyl acetate-methanol-water (HEMWat) two-phase s