呼吸道综合症病猪群病原检测与分析.pdfVIP

福建农业学报25( 2) : 131~ 134,2010 : 1008- 0384 (2010) 02- 131- 04 F uj ian Journal of A g ricultural Sciences 1 2 2 2 1 2 叶耀辉 , 吴南洋 , 黄梅清 , 郑 敏 , 雷松波 , 张世忠 ( 1., 355017;2. , 350013) : PCR : RT- PCR PRRSV , PRVCSFVPCV2 SIV ; RT-PCR ; Marc- 145 1 , , ; (PRRSV) : ; ; ( PRRSV) ; ; : S 8553 : A Pathogenic detection and identification of swine respiratory syndrome 1 2 2 2 1 2 E ao-hui , WU Nan-yang , HUANG Me-i qing , ZHENG Min , LEI Song-bo , ZHANG Shi-zhong ( 1. N ing deA gricul tural Science Research I nstitute of F ij ian Province , Fu an, Fuj ian 355017, China; 2. I nstitute of A nimal H us and ry and Veterinary M ed icine, Fij ian A cademy of A gricul tural S ciences, F uz hou, F uj ian 350013, China) Abstract: The study detected various kinds of pathogens in pigs suffering from respiratory syndrome by PCR technique and immunofluorescent test. The RT-PCR results showed that the tissue samples of lung and lymph nodes from diseased pigs were positive for PRRSV variant but negative for PRV, CSFV, PCV2 or SIV. A virus strains were also isolated from positive samples by inoculating it onto Marc- 145. The 30 days- old piglets inoculated with the isolates showed the same symptoms as appeared naturally on the diseased pigs in the clinic. The virus was recov- ered from and the interstitial Pneumonia observed in the diseased pigs. Thus, it was concluded that the respiratory disease outbreak in the swine farm was caused by a PRRSV variant. Key words: respiratory disease; swine; porcine reproductive and respiratory syndrome virus (PRRSV) ; pathogen detection; variant ( Porcine respiratory 80%, 10%~ 60% diseas