内参基因在昆虫实时荧光定量pcr中的研究进展-应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2016, 53(2): 237246. DOI: 10.7679/j.issn.20951353.2016.031 内参基因在昆虫实时荧光定量 * PCR 中的研究进展 史彩华1, 2** 张友军2*** 1. 434025 2. 100081 摘 要 PCR PCR qRT-PCR RNA 关键词 qRT-PCR Advances in reference gene for real-time quantitative reverse transcription PCR (qRT-PCR) of insects research SHI Cai-Hua1, 2** ZHANG You-Jun2*** (1. Yangtze University, Jingzhou 434025, China; 2. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract Real-time quantitative reverse transcription PCR (qRT-PCR) is one of the most commonly used technologies for genes expression analysis, with its high sensitivity, specificity, veracity, etc. In order to avoid differences of RNA quality and efficiency of reverse transcription, it is necessary to use housekeeping gene as the reference for data normalization. Some researches indicate that different reference genes should be selected regarding different experimental conditions and different insect species. Here, we summarized the advances in reference genes for qRT-PCR of insects research. This review provides a comprehensive understanding of selecting appropriate reference genes for qRT-PCR in insect molecular biological studies. Key words insects, reference gene, real-time quantitative reverse transcription PCR PCR Quantitative real-time PCRqRT-PCR Li et al.2015 Li et al. RNA 2013Northern-blot Suzuki et al.2000 Nolan