三羟基异黄酮对瘢痕疙瘩成纤维细胞增殖及-第三军医大学学报.docVIP

Genistein对人瘢痕疙瘩成纤维细胞增殖及侵袭作用的影响 曹 川，戴 霞，李世荣，李 丹，张 谊，冯 智，陈艳清 (第三军医大学：西南医院整形外科，重庆 400038) 提 要: 目的 观察57,4’-三羟基异黄酮(Genistein)对体外培养的人瘢痕疙瘩成纤维细胞生长及侵袭作用的影响，探讨其抗纤维化作用的。方法 以5、50、100μmol/L浓度Genistein处理体外培养的人瘢痕疙瘩成纤维细胞，MTT检测细胞增殖H3-脯氨酸掺入法检测细胞胶原合成，Transwell小室趋化运动模型检测细胞侵袭。结果 Genistein瘢痕疙瘩成纤维细胞的增殖及胶原合成，细胞的侵袭能力下降结论 Genistein具有体外抗瘢痕疙瘩纤维化与侵袭的作用，可成为治疗瘢痕及纤维化疾病的有效药物。 5,7,4’-三羟基异黄酮；瘢痕疙瘩；成纤维细胞；增殖；侵袭ffects of genistein on proliferation and invasion of human keloid fibroblasts CAO Chuan, DAI Xia, LI Shi-rong, LI Dan, ZHANG Yi, FENG Zhi, CHEN Yan-qing (Department of Plastic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China) Abstract: Objective To observe the effects of genistein on the proliferation and invasion of the cultured human keloid fibroblasts and explore the mechanism of its anti-fibrosis. Methods The human keloid fibroblasts were cultured and stimulated with 25, 50, 100 μmol/L genistein, respectively. Their proliferation was measured by MTT. The collagen synthesis was assessed by 3H-proline incorporation method. The invasion of human keloid fibroblasts was detected in Transwell chemotaxis chamber model. Results Genistein markedly inhibited the proliferation and collagen synthesis of the cultured keloid fibroblasts and the inhibitory effects were increased with the increasing concentration of genistein. Genistein also weakened the invasion of keloid fibroblasts. Conclusion Genistein has the ability of anti-fibrosis and inhibits the invasion of human keloid fibroblasts in vitro, indicating genistein may be an effective drug for pathological scar or fibrosis disease. Key words: genistein; keloid; fibroblast; proliferation; invasion 瘢痕疙瘩(eloid)是一种胶原等细胞基质过度沉积为特征的皮肤纤维化疾病具有侵袭性生长的特性。目前发病机制，也无满意的治疗药物手术切除极易复发5，7，4’－三羟基异黄酮(enistein)是一种特异性酪氨酸蛋白激酶(tyrosine protein kinase，TPK)抑制剂，对多种肿瘤和纤维化具有作用［5］本实验Genistein对瘢痕疙瘩增殖和作用的影响，为临床应用Genistein提供依据。苏州YJ-875型超净工作台、美国SHEL- LAB1815TC型二氧化碳培养箱、美国Beckman LS26500液体闪烁计数仪、美国BIO-RAD 550型酶标仪Transwell小室(滤膜为μm孔径的无聚碳酸脂聚乙烯吡咯烷酮多孔滤膜