巴西橡胶树importin和exportin基因的克隆及其乙烯响应规律分析.pdfVIP

热带亚热带植物学报 2016, 24(3): 243 ~ 251 Journal of Tropical and Subtropical Botany 巴西橡胶树importin 和exportin 基因的克隆及其乙 烯响应规律分析 冯伟强，仝征，靳翔，王丹，孙勇，蒙雪茹，王旭初* (1. 海南大学农学院，海口570228；2. 中国热带农业科学院热带生物技术研究所，海口571101) 摘要：为了解巴西橡胶树(Hevea brasiliensis) 中importin 和exportin 基因的功能，采用RACE 技术从巴西橡胶树中克隆了他们 的cDNA 全长，分别命名为HbIMP 和HbXPO 。结果表明，HbIMP 全长4170 bp ，编码1115 个氨基酸；HbXPO 全长4108 bp ， 编码1081 个氨基酸。HbIMP 和HbXPO 分别与importin5 和exportin1A 亚家族的同源性最高。HbIMP 包含18 个外显子，17 个内含子，而HbXPO 包含30 个外显子，29 个内含子，且在上游调控序列中都存在乙烯响应元件ERELEE4 和LECPLEACS2 。 在无乙烯刺激情况下，HbIMP 和HbXPO 在根、茎、叶、皮中有表达，但在胶乳中几乎检测不到表达；乙烯处理后HbIMP 和HbXPO 在胶乳中被明显诱导表达。因此，推测HbIMP 和HbXPO 参与了乙烯诱导蛋白出入细胞核的转运。 关键词：巴西橡胶树；乙烯；核质转运蛋白；基因克隆 doi: 10.11926/j.issn.1005-3395.2016.03.001 Molecular Cloning of importin and exportin in Hevea brasiliensis and Gene Expression Induced by Ethylene * FENG Wei-qiang, TONG Zheng, JIN Xiang, WANG Dan, SUN Yong, MENG Xue-ru, WANG Xu-chu (1. College of Agronomy, Hainan University, Haikou 570228, China; 2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences , Haikou 571101, China) Abstract: The aim was to understand the functions of importin and exportin genes in Hevea brasiliensis . The full-length cDNAs of importin and exportin gene were cloned from H. brasiliensis by rapid amplification of cDNA ends (RACE) technique, named as HbIMP and HbXPO, respectively. The results showed that HbIMP was 4170 bp in length, encoding 1115 amino acids, and HbXPO for 4108 bp in length, encoding 1081 amino acids. Multiple sequence alignment and phylogenetic analysis revealed that HbIMP belonged to importin5 subfamily, while HbXPO to exportin1A subfamily. The sequences analysis showed that HbIMP contained 18 exons and 17 introns, while HbXPO had 30