广藿香青枯病菌的分离培养及致病性测定.pdfVIP

• 1596 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 8 期 2011 年 8 月 广藿香青枯病菌的分离培养及致病性测定 * 刘 丹，贺 红 ，黄海波，谢建辉，柴婷婷 广州中医药大学中药学院，广东 广州 510006 摘 要：目的 分离广藿香青枯病病原菌 Ralstonia solanacearum ，了解其细菌学性状，并进行致病性测定，为广藿香青枯病 的防治提供依据。方法 在 TZC 培养基 [蛋白胨 10.0 g 、酸水解酪蛋白 1.0 g 、TTC （2 ，3，5-氯化三苯基四氮唑）0.05 g、 葡萄糖 10.0 g 、H2O 1 L，pH 6.8～7.0] 上划线分离培养广藿香青枯菌；通过 3 糖 3 醇试验对各菌株的生化型进行划分；采 用伤根浸泡接种法对分离所得菌株进行致病性测定。结果 经分离纯化，得到在细菌学性状上有明显差异的 7 个青枯菌菌株。 其中 HX5 、HX7 为生化型 I ；HX1 、HX6 为生化型 II ；HX2 、HX4 为生化型 III ；HX3 为生化型 V 。致病性试验表明，大部 分青枯菌菌株表现较强的致病性，其中以菌株 HX4 和 HX5 致病力强且致死率高。结论 广藿香青枯病菌存在多个生理小种， 在菌体形态、生化分型和致病性等方面均有一定的差异。 关键词：广藿香；青枯病菌；病原菌；TZC 培养基；致病性测定 中图分类号：R282.22 文献标志码：A 文章编号：0253 - 2670(2011)08 - 1596 - 04 Isolation of pathogenic Ralstonia solanacearum from Pogostemon cablin and determination of its pathogenicity LIU Dan, HE Hong, HUANG Hai-bo, XIE Jian-hui, CHAI Ting-ting College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China Abstract: Objective To isolate the pathogenic Ralstonia solanacearum from the infected Pogostemon cablin plants and to study the bacteria characteristics, so as to lay a foundation for the control of bacterial wilt. Methods Pathogenic strains of R . solanacearum were isolated from the infected P . cablin plants by being streaked on a tetrazolium chloride (TZC) medium. These strains were used for the classification based on oxidative utilization of three kinds of disaccharide (lactose, maltose, and cellobiose) and three kinds of hexy I alcohol (mannitol, dulcitol, and sorbitol). The pathogenicity was tested by the methods of cutting and soaking roots. Results Seven strains of pathogenic R. solanacearum were isolated from the infected P . cablin plants which contain four biotypes. Strains of HX5 and HX7 belong to biotype I, HX1 and HX6 belong to biotype II, HX2 and HX4 belong to biotype III, and HX3 be