野大豆等宿主根瘤菌表型及16srdnapcr-rflp研究-安徽农业大学.pdfVIP

安徽农业大学学报, 2012, 39(2): 273-279 Journal of Anhui Agricultural University 网络出版时间：2012-02-29 14:22 [URL] /kcms/detail/34.1162.S1422.026.html 野大豆等宿主根瘤菌表型及 16S rDNA PCR-RFLP 研究 王 静，刘 磊，梁 芳，欧明丽，刘少佳，胡玉莹，杨 帆，韩素贞* (首都师范大学生命科学学院, 北京 100048) 摘 要：采用数值分类和16S rDNA PCR-RFLP对分离自北京部分地区野大豆（Glycine soja sieb ）、大豆（Glycine max ）、菜豆（Phaselous vulgaris ）和长萼鸡眼草（Kummerowiae stipulacea ）等宿主的60 株菌及 10 株根瘤菌参比菌 株进行了研究。数值分类结果表明，在70.5%相似性水平上，所有的菌株可分为3 群：群I 为未知菌群，群II 为快 生和中慢生菌，群III 为慢生菌群。依据16S rDNA PCR-RFLP 分析建立的树状图，在69 ％的相似性水平上所有的 供试菌株可以分为9 个系统发育分支。分支I 、V 、VI 、VII 、VIII 和IX 没有参比菌，数值分类中群I 的供试菌株 基本上都处于这些分支。分支II 为Sinorhizobium-Mesorhizobium-Rhizobium ，分支III 为Agrobacterium 分支，分支 IV 为Bradyrhizobium 分支。 关键词：根瘤菌；数值分类；16S rDNA； PCR-RFLP 中图分类号：Q939.11 文献标识码：A 文章编号：1672−352X (2012)02−0273−07 Phenotypic analysis and 16S rDNA PCR-RFLP of rhizobial strains isolated from Glycine max etc. WANG Jing, LIU Lei, LIANG Fang, OU Ming-li, LIU Shao-jia, HU Yu-ying, YANG Fan, HAN Su-zhen (Life Science College, Capital Normal University, Beijing 100048) Abstract: Sixty strains obtained from root nodules of Glycine soja sieb, Glycine max, Phaselous vulgaris and Kummerowiae stipulacea of Beijing were studied with numerical taxonomy and 16S rDNA PCR-RFLP. Results of numerical taxonomy indicated that all strains included 10 rhizobium reference strains were divided into 3 groups at 70.5% similarity. Group I is an unknown group with no reference strains. Group II belongs to fast and mid- dle-slow-growing type, and group III belongs to slow-growing kind. The dendrogram derived from 16S rDNA PCR-RFLP showed that all strains divided into nine phylogenetic branches at the similarity of 69 ％. They are branches I,