材料与方法-实用皮肤病学杂志.docVIP

投稿文章格式要求 中文标题：四号宋体加粗，2.5倍行距） 英文标题：四号、字体TIMES NEW ROMAN 作　　者：小四号楷体 正　　文：小四号宋体（中文），小四号TIMES NEW ROMAN(英文)，1.5倍行距 中英文摘要：5号宋体（中文），5号TIMES NEW ROMAN(英文)，1.5倍行距 图标和图表的文字是五号宋体 参考文献：小5号宋体，单倍行距 范例： 光动力学疗法对豚鼠光老化治疗作用的研究 严淑贤，王　艳，卢　忠，钱　辉 【摘要】目的 探讨5-氨基酮戊酸（ALA）联合强脉冲光（IPL）的光动力学疗法（PDT）对豚鼠光老化模型的影响，并摸索合适的ALA治疗浓度。方法 以模拟日光光源（SSR）建立豚鼠光老化模型，10%或20%ALA联合IPL治疗，每3周一次共3次。结果 豚鼠以亚红斑量SSR照射13周，光照部位真皮浅层出现了典型的“光化性弹力纤维变性”的损害。经10%ALA联合IPL治疗后，上述改变明显改善，而20%ALA联合IPL可进一步加重损伤。结论 光动力疗法对光老化有一定改善作用，选择合适的ALA治疗浓度很重要。 【关键词】 紫外线；皮肤衰老；5-氨基酮戊酸；强脉冲光；光动力学疗法 Study of photodynamic therapy on guinea pig photoaging model YAN Shu-xian，WANG Yan, LU Zhong, QIAN Hui. Department of Dermatology，Huashan Hospital of Fudan University，Shanghai 200040，China [Abstract] Objective To evaluate the efficacy of 5-aminolevulinic acid (ALA) with intense pulsed-light (IPL), known as photodynamic therapy (PDT) on guinea pig photoaging model and to set up the proper concentration of ALA. Methods The guinea pig photoageing model with chronic sun-simulating radiation (SSR) exposure was set up. ALA at the concentration of 10% or 20% was used and the animals received a series of 3 treatments 3 weeks apart. Dermal structure, including elastic and collagen fiber was observed. Results Albino guinea pigs were subjected to suberythemal dose of SSR three times a week. Thirteen weeks after exposure, there was typical “solar elastosis” damage in the upper dermis. ALA at concentration of 10% with IPL improved the photodamage changes, while ALA at concentration of 20% with IPL worsened the photodamage signs. Conclusion PDT has rejuvenation effects on photoaging. It is important to choose proper concentration of ALA. 【Key words】 Ultraviolet ray；Skin ageing；5-aminolevulinic acid；Intense pulsed-light；Photodynamic therapy 皮肤长期受到紫外线照射，会产生光老化，出现皮肤粗糙晦暗，密集的细纹与皱纹，点状色素沉着、红斑和毛细血管扩张等改变，组织病理上表现为真皮中上层特征性的光化性弹力纤维变性[1]。臭氧层被破坏使到达地球表面的紫外线逐年增加，光老化问题日益引起关注。近年来，以强脉冲光（IPL）为代表的非剥脱性嫩肤治疗在临床上广泛的开展，并取得一定的疗效[2]。有临床研究显示，外用光敏剂5-氨基酮戊酸（ ALA ）后照射IPL的光动力疗法（PDT），比单纯的IPL治疗面部光老化有更好的疗效[