恒定磁场对骨组织中细胞影响的研究.PDF

　2006 8 12 4 　Chin J Osteoporos,August 2006,Vol 12, No.4 333 ·· 张小云　张宇 :　 , 。 　 、, 0.38 T、0.48 T , 、。 　 , 、, 、。 　 , , 。 :;;;; Effect of permanent-magnetic field on cells in bone　ZHANGX iaoyun, ZHANG Yu.College of Biotechnology, Microorganism and Gene Engineering Vital Laboratory of henzhen University, henzhen 　518060, China Abstract:Objective 　To investigate the effect of permanent-magnetic field on the gro th and function in osteoblast and osteoclast and its effect on bone marro stromal cells differentiation into osteoblast in order to clarify the therapeutic mechanism of osteoporosis.Methods　After exposure to 0.38 T and 0.48 T permanent-magnetic field on primary in vitro cultured osteoblast, osteoclast and bone marro stromal cells, the effects of gro th, differentiation and functions ere observed.Results　The differentiation of bone marro stromal cellsinto osteoblast as promoted by the permanent-magnetic field exposure, and the proliferation, differentiation and function of the osteoblast as also improved, but the gro th, differentiation and function of the osteoclast as suppressed. Conclusions　Permanent-magnetic field could accelerate bone formation and inhibit bone absorption, thus being the partial mechanism for osteoporosis management. Key words:Permanent-magnet field;Necrosis, femoral head;Marro stromal cells;Osteoblast;Osteoclast; Osteoporosis 　　 , 《》, , 。 。 　　 、、。、 、 1　 、。 。 1.1　 , 3 d 4 ～6 SPF KM ( [1] );, -70 ℃ , , ;(); [2] α-MEM (Gibco);、1α-25 , 。 , D3、、、、 TRAP N-2--N-2- [3] (HEPES)Sigma。MTT、、 C、β- 。 、, 、、、 Ⅱ (Sigma)、(ALP)、 :518060