二次正交旋转组合设计优化药桑花青素提取工艺.pdfVIP

※工艺技术 食品科学 2012, Vol. 33, No. 08 33 1 , 2 2 2 1 1 , 2 , 杨 松 ，周蓓蓓 ，程江华 ，邬德轩 ，丁之恩 * ，闫晓明 * (1.安徽农业大学茶与食品学院，安徽 合肥 230036 ；2.安徽省农业科学院农产品加工研究所，安徽 合肥 230031) 在超声波辅助条件下，采用四元二次正交旋转组合设计研究乙醇体积分数、液料比、时间、温度 4 个 因素对药桑花青素提取效率的影响，用DPS7.05 软件分析得出 4 个因素的回归方程。结果表明，超声波辅助提取 最佳工艺条件为：乙醇体积分数范围 52.46%～55.93%、提取液料比范围39.51:1～42.43:1(mL/g) 、提取时间范围 27.77～32.23min 、提取温度范围50.18～52.4 ℃，在此工艺条件下花青素提取效率均能达到67% 以上。 药桑；花青素；正交旋转组合设计；提取；优化 Optimization of Anthocyanidin Extraction from Black Mulberry (Morus nigra L.) by Quadratic Orthogonal Rotation Combination Design 1,2 2 2 1 1, 2, YANG Song ，ZHOU Bei-bei ，CHENG Jiang-hua ，WU De-xuan ，Ding Zhi-en *，YAN Xiao-ming * (1. College of Tea and Food, Anhui Agricultural University, Hefei 230036, China ； 2. Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China) Abstract ：A quadratic orthogonal rotation combination design was used to explore the effects of ethanol concentration, material-to-liquid ratio, extraction time and extraction temperature on extraction rate of anthocyanidin from black mulberry (Morus nigra L.) by ultrasonic-assisted extraction. A regression equation was established using DPS7.05 software. The optimal extraction conditions were found to be 52.46% －55.93% ethanol as extraction solvent at a liquid-to-material ratio of 39.51:1 － 42.43:1 (mL/g) for 27.77 －32.23 min of extraction at 50.18－52.4 ℃. Under these conditions, the extraction rate of anthocyanidin was more than 67%. Key words ：black mulberry ；anthocyanidin ；orthogonal rotation combination design ；extraction ；optimizati