卡氏肺孢子菌肺炎模式识别受体dectin-1tlr-第三军医大学学报.docVIP

卡氏肺孢子菌肺炎鼠肺模式识别受体Dectin-1、TLR-2和TLR-4的表达变化* 郑玉强1 刘爱波1 蒲 瑜1 蔡 辉2 叶 彬2 (1.重庆医科大学附属儿童医院临检中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室，重庆市儿童发育重大疾病诊治与预防国际科技合作基地，重庆，400014；2.重庆医科大学病原生物教研室，重庆，400016) 摘要：目的 研究卡氏肺孢子菌(Pneumocystis carinii, Pc)肺炎鼠肺Dectin-1、TLR-2和TLR-4的表达，探讨模式识别受体表达改变与PCP疾病发生的相互关系。方法 实验分4组：正常对照组、Pc刺激组、PCP模型组以及PCP模型恢复组。地塞米松磷酸钠免疫抑制方法建立PCP动物模型，改良四胺银（GMS）染色检测Pc包囊；肺组织切片HE染色观察肺组织病理变化；实时荧光定量和Western-blot检测 Dectin-1、TLR-2和TLR-4的mRNA以及蛋白的表达。结果 Pc刺激组的Dectin-1、TLR-2以及TLR-4的mRNA以及蛋白的表达明显高于正常对照组（P0.05）；Pc刺激组和PCP恢复组Dectin-1的mRNA以及蛋白显著高于PCP组（P0.05），而肺部Pc载荷量显著低于PCP组（P0.05）。结论 对免疫功能正常宿主，Dectin-1 TLR-2和TLR-4受体可能在防Pc感染中起重要作用；地塞米松抑制了鼠肺Dectin-1、TLR-2和TLR-4受体的表达，这可能与PCP疾病的发生和发展有关。 关键词：卡氏肺孢子菌；Dectin-1；TLR-2；TLR-4；大鼠模型 中图法分类号：R379.9;R392.9 Expression of Dectin-1、TLR-2 and TLR-4 in Pneumocystis carinii pneumonia rat* ZHENG Yuqiang1, LIU Aibo1, PU Yu1, CAI Hui2, Wu Weihua2, YE Bin2 (1.Laboratory Medicine, Childrens Hospital of Chongqing University of Medical Sciences, Ministry of Education Key Laboratory of Child Development, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperration Center for Child Development and Disorders, Chongqing 400014,China；2.Department of Pathogen Biology, Chongqing University of Medical Sciences, Chongqing 400016,China) ABSTRACT：Objective To study the expression of Dectin-1、TLR-2 and TLR-4 in Pneumocystis carinii pneumonia (PCP) rat, and to explore the influence of dexamethasone on the expression of Dectin-1、TLR-2 and TLR-4 and its relationship with progression of PCP. Methods Rat were divided into four groups which were normal control, treated with P.carinii (Pc), PCP model and recovered PCP model. PCP rat were induced with dexamethasone in this study, and Pc cysts were detected by GMS stains. Real-time PCR and Western blot were utilized to detected the expression of Dectin-1,TLR-2 and TLR-4 in lung. Results The expression of Dectin-1,TLR-2 and TLR-4 were much higher in the treated group with Pc than that of the normal