基因芯片与植物基因差异表达分析-植物研究.pdfVIP

22 　3 　　　　　　　　　　　　　　　　　　　　　　 2002 　7 Vol.22 　No.3　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH July, 　2002 1, 2 1 李同祥 　王进科 (1., 　210096) (2., 　730030) 　　基因芯片为研究植物不同个体或物种之间以及同一个体在不同生长发育阶段、正常和疾 状态下基因表达的差异、某 一性状多基因的协同作用, 寻找和定位新的目的基因等方面带来了 革命性的变革。与传统研究基因差异表达的方法相比, 它具有微型化、用材少、快速、准确、灵敏度 能高基、在因同等一研究方面已取得了显著的成绩, 如拟南芥、酵母、水稻等。 　基因芯片;差异表达;植物 DNA MICROARRAY AND ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN PLANT 1, 2 1 LI Tong-xi ng 　WANG Jin-ke (1.N tion l L bor tory of Molecul r nd Biomolecul r Electronics, Southe st University ,N njing 210096) (2.G nsu R dio TV University, L nzhou 730030) Abstract 　DNA micro rr y technology h s m de revolution ry tr nsform tion in studying differenti l gene expression, the coll bor tion of multigene for one tr it, discovering nd loc ting t rget genes, mong different individu l in s me or different species nd one pl nt individu l in different development st ge s well s be- tween norm l nd dise sed condition .The m jor dv nce of DNA micro rr y technology, s comp red to con- vention l techniques, results from the sm ll size of the rr y, which llows for higher ccur te, quicker process nd higher sensitivity en bles the p r llel screening of l rger number of genes simult neously on one chip nd provides the opportunity to use sm ller mounts of st rting m teri l.M ny signific nt chievements h ve been obt ined in picturing the expression profiles of genes, discoverying the t rget genes nd elucid ting gene functions with DNA micro rr y on pl nt, such s Arabidopsis nd ye st, rice. Key words　DNA micro rr y;differenti