烟草wrky8基因的克隆与表达分析-湖南农业大学学报欢迎您！.pdfVIP

38 3 () Vol.38 No.3 2012 6 Journal of Hunan Agricultural University (Natural Sciences) Jun2012 DOI:10.3724/SP.J.1238.2012.00256 烟草 WRKY8 基因的克隆与表达分析 1,2 1,2 1* 1,2* (1. 410128 2. 410128)   摘 要RT–PCR WRKY8 1 551 bp 516 2 WRKY C H (C–X –C–X –H–X –H) 2 2 4–5 22–23 1 RT–PCR NtWRKY8 关 键 词WRKY8 中图分类号Q786 文献标志码A 文章编号1007–1032(2012)03–0256–06 Cloning and expression analysis of WRKY8 gene in Nicotiana tabacum MEI Hua1,2, YANG Hui1,2, TIAN Yun1*, LU Xiang-yang1,2* (1.Hunan Agricultural Bioengineering Research Institute, Changsha 410128, China; 2.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China)   Abstract In this paper, the NtWRKY8 gene was cloned from Nicotiana tabacum L. based on the database resources and RT–PCR technology. Sequence analysis showed that NtWRKY8 gene contained a complete ORF, which was 1 551 bp and encoded a protein of 516 amino acid residues. The WRKY8 protein had two conserved WRKY domains, and the zinc-finger structure was C H (C–X –C–X –H–X –H). Functional analysis indicated that this protein was probably 2 2 4–5 22–23 1 located in the nucleus and had functions in replication, transcription and regulatory, and consequently could regulate the growth, development and response to stress in plants. The RT–PCR experiment showed that the relative expression of NtWRKY8 gene was induced under low-