微粒体甘油三脂转运蛋白mtp的研究进展-生物技术通报.pdfVIP

生物技术通报 BIOTECHNOLOG Y B ULLETIN 2005 1 MTP * 叶健强 王继文 ( , 625014) : 微粒体甘油三酯转运蛋白MTP(microsomal triglyceride transfer protein, MTP) 首先是从牛的肝细胞微粒 体碎片中分离 得的, 其作用是加速甘油三脂( triglyceride , TG) 胆固醇( c olesteryl ester , CE) 和磷脂酰胆碱( p os- [1] p atidylc oline , PC) 的转运和细胞或亚细胞膜的生物合成它后来在肝细胞和小肠的微粒体膜中发现 , 由于它的 位置及其转运TG 可以推测与血浆脂蛋白中极低密度脂蛋白( very low density lipoprotein , VLDL) 和乳糜微粒( c ylomi- crons , CM) 的组装过程有关 : 微粒体甘油三脂转运蛋白 脂蛋白 Apo B Recent Advance On Microsomal Triglyceride Transfer Protein * Ye Jianqiang Wang Jiwen ( college of animal science SichuanA gricul ture universi ty , yaan 625014) A bstract : T e microsomal triglyceride transfer protein ( MTP) w ic was originally isolated from t e microsomal fraction of bovine liver , accelerates t e transport of triglyceride(TG) , c olesteryl ester ( CE) and p osp atidylc oline ( PC) between synt et- ic membranes. T e protein was subsequently found wit in t e lumen of microsomes isolated from bot liver and intestine. T e tis- sue distribution wit its ability to transport TG , led to t e ypot esis t at MTP is involved in t e assembly of plasma lipoprotein , very low density lipoprotein (VLDL) and c ylomicrons ( CM ) . Key words: Microsomal triglyceride transfer protein( MTP) Lipoprotein Apob 1 , PDI MTP , PDI ( c aperone) PDI , 0. 5%~ 1. 0% MTP , MTP MTP SDS Sf9,MTP , PDI MTP 58 000 , MTP PDI 491 88 000[ 2] ; , MTP 150 000, MTP 1/ 10, , , 35~ 40 379~ 384 ( protein disulfide isomerase , MTP ; PD