基于pcr技术的玉米丝轴黑粉菌侵染率及扩展-中国科技论文在线.pdfVIP

中国科技论文在线 中国农业科学 2006,39(9):1804-1809 Scientia Agricultura Sinica 基于PCR 技术的玉米丝轴黑粉菌侵染率及扩展进程的研究 1 华中农业大学作物遗传改良国家重点实验室 国家分子技术育种中心 武汉 2 北京市农林科学院玉米研究中心 北京 430070 , 100089 摘要 关键词 The Research of Sporisorium reiliana’s Infection Efficiency and Spreading in Maize Based on PCR Assays 1 1 2 2 1 1 NI Shen , XIAO Yan-nong , WANG Feng-ge , ZHAO Jiu-ran , ZHANG Shao-peng , ZHENG Yong-lian 1 National Key Laboratory of Crop Genetic Improvement National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070; 2 Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089 Abstract: Objective Head smut of maize substantially reduces grain yield. Here we demonstrate a recent survey of the rate of infection of head smut by PCR. Method Using gene-specific primer derived from the head smut genomics and maize temporal and tissue-specific DNA, the invading process of the pathogen is displayed clearly. Result The study shows that the differential resistance of maize against head smut depends mainly on the ability of resistance against infection and spreading. Conclusion The above effect disappears in the stamen when the tumor forms during tasseling stage. Key words: Maize; Head smut; PCR; Infection; Spread 的玉米幼苗进行侵染 侵染后菌丝主要随着生长锥延 0 引言 伸[6] 被侵染的玉米植株在早期并无明显症状 某些 本研究的重要意义 玉米丝黑穗病 head 玉米幼苗可在第四叶和第五叶叶脉出现退绿小斑点 smut 是玉米生产中的重要病害 在很多国家均有不 但最为直观明显的症状是开花期雌雄穗产生充满孢子 同程度发生