食管鳞状细胞癌中EZH2的表达对患者术后预后的-南方医科大学学报.PDFVIP

J South Med Univ, 2013, 33(1): 99-102 doi 10.3969/j.issn. 1673-4254.2013.01.21 ·99 · 临床研究 食管鳞状细胞癌中食管鳞状细胞癌中EZHEZH22 的表达对患者术后预后的影响的表达对患者术后预后的影响 王浩然，王铭辉，连贵勇，吴多光 中山大学孙逸仙纪念医院心胸外科，广东 广州 510120 摘要：目的 通过研究鳞状细胞癌EZH2 蛋白的表达，探讨其表达程度高低与患者术后预后的关系。方法 对1996~2006 年本院 心胸外科食管鳞癌术后病人随访，纳入研究对象，采用免疫组化方法研究其中鳞癌组织（102例）、癌旁组织（30例）、正常组织（30 例）中EZH2 蛋白的表达情况，研究该蛋白表达与临床病理参数（性别、年龄、分化、分期、淋巴结转移）的关系；结合随访资料，采 用生存分析研究蛋白表达高低对术后患者预后的影响。结果 食管鳞状细胞癌中的EZH2 表达高于非癌组织，中低分化鳞癌组 织表达高于高分化组织，有淋巴结转移组织表达高于无转移组织，临床TNM 分期Ⅱ、Ⅲ期患者表达高于Ⅰ期患者，低于Ⅳ期患 者，高表达组患者术后远期生存时间较低表达组明显降低（P0.05）。结论 EZH2 促进食管鳞癌的淋巴结转移，降低分化程度。 术后患者EZH2 高表达组较低表达组预后差。 关键词：食管鳞状细胞癌；EZH2 ；免疫组化；预后 Expression of enhancer of zeste homolog 2 in esophageal squamous cell carcinoma and its prognostic value in postoperative patients WANG Haoran, WANG Minghui, LIAN Guiyong, WU Duoguang Department of Cardiothoracic Surgery, Sun Yat-sen Memorial Hospital, Guangzhou 510120, China Abstract: Objective To investigate the expression of enhancer of zeste homolog 2 (EZH2) in esophageal squamous cell carcinoma and its association with the prognosis of postoperative patients. Methods Surgical specimens were obtained from 102 patients with esophageal squamous cell carcinoma undergoing radical resection in our hospital from 1996 to 2006. Immunochemistry was employed to examine EZH2 protein expressions in the specimens, including 102 carcinoma tissue specimens, 30 adjacent tissue specimens and 30 normal esophageal tissue specimens. The expression levels of EZH2 were analyzed in relation to the clinicopathological parameters of the patients including gender, age, tumor differentiation, TNM, and lymph node metastasis. The postoperative patients were followed up to analyze the association of EZH2 expression with the clinical outcomes. Results The esophageal squamous cell carcinoma tissue showed a higher EZH2 expression tha