用双向电泳分析百合减数第一分裂周期蛋白质的变化-植物研究.pdfVIP

21　4 　　　　　　　　　　　　　　　　　　　　　　 2001　10 Vol.21　No.4　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Oct., 　2001 王振英　彭永康　陈　宏 (, 　300074) 　　利用双向电泳方法检测到百合减数第一分裂花粉母细胞内约有 130 种蛋白质 分, 其 中有两类蛋白质呈现周期性变化。70KD/pI6.1、66KD/pI6.4、68KD/pI7.2在中期、后期消失, 末 期重新出现;而10KD/pI5.3则在中、后期出现, 末期消失。在减数第一分裂不同时期也有多种 蛋白质的合成与降解现象, 20 KD/pI3.7、17 KD/pI3.8、16 KD/pI3.7、15 KD/pI3.3、11KD/pI3. 8、10 KD/pI3.7六种蛋白质在后期合成;蛋白质呈现出周期变化和在不同分裂时期的合成与降 解可能与减数分裂不同时期的周期调控有关。 　减数第一分裂;双向电泳;细胞周期;调控;百合 STUDIES ON CHANGE OF PROTEINS AT DIFFERENT FIRST MEIOTIC PHASES IN LILI TUM DA VIDILL DUCH USING 2D SDS ANG Zhen-ying　PENG Yong-kang　CHEN Hong (Department of Biology Tian Jin Normal University, TianJin 300074) Abstract 　More than 130 protein species have been identified in the first meiotic phase of pollen mother cells in L il ium david ii Duch.Comparing the proteins change during the different phase it wasshown that different patternsof cyclic changes occurred in various proteins.70 KD/pI6.1, 66 KD/pI6.4, 68KD/pI7.2, were disappearedat metaphase, anaphase and were appeared againat the telophase, protein specie, 10 KD/pI5.3, however, was appeared at metaphase, anaphase and was disappeared at telophase.Syntheticand degradationof someprotein specieswere also observedduring the different first meiotic phase.Protein species of 20 KD/pI3.7, 17 KD/pI3.8, 16 KD/pI3.7, 15KD/pI3.3, 11KD/pI3.8, 10 KD/pI3.7weresyntheticed at anaphase.Itwas thought that there are the relationshipbetween the protein cyclic change, syntheticdegradation at different first meiotic phasesand meiotic cyclic regulation. Key words　first meiosis;2D SDS;cell cycle regulation;L ili um david ii Duch 　　Howand1953, , G1