褐飞虱翅型分化的分子机制.pptVIP

Two insulin receptors determine alternative wingmorphs in planthoppers  Hypothesis: wing morph determination in BPHs may be regulated by a nutrient-sensing pathway . The insulin/insulin-like growth factor (IGF) signalling (IIS) pathway is an evolutionarily conserved nutrient-sensing pathway thatmodulates growth and development in metazoans. BPHs have two insulin receptors, N.lugens(Nl)InR1 and NlInR2 . Knockdown of NlInR2 (dsNlInR2) led to a strong bias towards long-winged morphs compared to BPHs that were either not subjected to treatment(ck) or treated with double-stranded RNA(dsRNA) targeting the gene encoding green fluorescence protein (dsgfp) (Fig. 1c)  Knockdown of NlInR2 (dsNlInR2) led to a strong bias towards long-winged morphs compared to BPHs that were either not subjected to treatment(ck) or treated with double-stranded RNA(dsRNA) targeting the gene encoding green fluorescence protein (dsgfp) (Fig. 1c) knockdown of NlInR1 (dsNlInR1) produced the opposite response,inducing the development of short-winged BPHs(Fig. 1c) These findings indicate that expression of the two insulin receptors,NlInR1 and NlInR2, is tightly correlated with wing morph determination in BPHs, and that they have fully opposite roles in the process.  Silencing of NlChico or NlAkt phenocopied dsNlInR1. By contrast, silencing of NlFoxo or NlPten phenocopieddsNlInR2 . NlLnk was probably irrelevant to wing morph determination, suggesting that NlLnk is either dispensable or functionally complemented by NlChico.  Next examined whether NlInR2 shares a common signalling cascade with NlInR1 . Silencing of NlInR1, NlAkt or NlChico redirected development of dsNlInR2-treated BPHs from the long-winged to short-winged morphs (Fig. 2 a-e). Suppression of NlInR1–NlPI(3)K–NlAkt signalling is epistatic to the dsNlInR2 effect . DsNlInR2 but not dsNlInR1 strongly augmented the P-NlAkt level . Given the high sequence similarity between NlInR1 and NlInR2, we then asked whether NlInR2 c