超高效液相色谱快速测定卷烟主流烟气中7种挥发酚 fast analysis of 7 volatile phenols in mainstream cigarette smoke with ultra performance liquid chromatography.pdfVIP

烟草化学／TobaccoChemistry 烟草科技／TobaccoScience＆Technology 超高效液相色谱快速测定卷烟主流烟气中7种挥发酚 黄朝章 福建中烟工业公司技术中心，福建省厦门市海沧区新阳工业区新阳路I号 361022 关键词：超高效液相色谱；主流烟气；挥发酚；口一环糊精 摘要：为快速准确地测定卷烟烟气中的对一苯二酚、邻一苯二酚、间一苯二酚、苯酚、对一甲酚、邻一甲酚和 间一甲酚，建立了同时测定卷烟主流烟气中7种挥发酚的超高效液相色谱(UPLC)方法。采用1％醋酸水 UPLCBEH 溶液对捕集烟气粒相物的剑桥滤片超声萃取20 C。。色谱柱分 min，萃取液过滤后通过Acquity 离，以水相中含7．0×10。mol／L届一环糊精(口一CD)的流动相进行梯度洗脱，采用荧光检测器进行分析。该 间9min。该法适合批量卷烟主流烟气中7种挥发酚的快速分析。 中图分类号：TS411．2文献标识码：B文章编号：1002—0861(2011)08—0044—04 Fast of7VolatilePhenolsinMainstreamSmokewithUltraPerformance Analysis Cigarette Liquid Chromatography HUANGCHAO．ZHANG CenterofChinaTobaccoIndustrial Technology Fujian Corporation，Xiamen361022，Fujian，China Ultra Mainstream smoke；Volatile Keywords：performanceliquidchromatography； cigarette phenol； 口-Cyclodextrin orderto and determinethe Abstract：Inrapidlyaccurately and3一cresolin methodfor thesevenvolatile cresol，2一cresol smoke，a cigarette simultaneouslydetermining inmainstreamsmokewithultra phenols cigarette performanceliquidchromatography(UPLC)wasdeveloped．The total mattercollectedon filter wasextractedwithl％aceticacidunderultrasonicfor particulate Cambridgepad filtrated aWaters 20min．Theextractwas and UPLCBEHC,scolumnwith elution separatedby Acquity gradient mobile ofwater 7．0×10。3 recoveriesoftheseven using phase containing mol／L卢-cyclodextrin(卢-CD)．The from90％to102％withRSDof1．15％to thelimitofdetectionwasO．04to phenolsranged 5．79％，and 0．35“g／ timewas9minutes．Themethodwassuitableforthefa