issr分子标记鉴定陆川猪及其肉制品 species authentication of luchuan pig meat and meat products using issr molecular markers.pdfVIP

10 2014, Vol. 28, No. 10 肉类研究 中国肉类食品综合研究中心 分析检测 MEAT RESEARCH CHINA MEAT RESEARCH CENTER ISSR分子标记鉴定陆川猪及其肉制品 1 1,* 2, * 1 1 何瑜林 ，黄 丽 ，封 毅 ，王 淼 ，滕建文 （1.广西大学轻工与食品工程学院，广西 南宁 530004 ；2.广西中医药大学基础医学院，广西 南宁 530001 ） 摘  要：采用ISSR分子标记对陆川猪及其肉制品进行鉴伪识别，利用ISSR技术，对纯种陆川猪、陆川猪杂交品种 （二元杂猪和三元杂猪）及广西本地的巴马香猪和环江香猪的DNA指纹图谱进行分析。结果表明：从34 条引物中 筛选出3 条带型清晰、重复性好的引物WM12 、WM27 、WM10 ，其中引物WM12可用于鉴别陆川猪、二元杂猪和三 元杂猪的生鲜样品及腊肉制品，引物WM27可用于鉴别陆川猪和巴马香猪的生鲜样品及腊肉制品，引物WM10用可 于鉴别陆川猪和环江香猪的生鲜样品及腊肉制品。ISSR技术可快速准确的鉴定陆川猪、陆川猪杂交品种、巴马香 猪、环江香猪及其肉制品。 关键词：陆川猪；分子标记技术；ISSR Species Authentication of Luchuan Pig Meat and Meat Products Using ISSR Molecular Markers 1 1,* 2, * 1 1 HE Yu-ling , HUANG Li , FENG Yi , WANG Miao , TENG Jian-wen (1. Institute of Light and Food Engineering, Guangxi University, Nanning 530004, China; 2. Faculty of Preclinical Medicine, Guangxi University of Chinese Medicine, Nanning 530001, China) Abstract: In this study, species identification of Luchuan pig meat and meat products was carried out by DNA fingerprint profiling of meats from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs using ISSR (inter-simple sequence repeat) molecular markers. The results showed that 3 clear and repeatable primer pairs WM12, WM27 and WM10 were selected from 34 primers. WM12 was useful for discriminating fresh and cured meat from purebred Luchuan and hybrid Luchuan pigs, while Luchuan pigs could be distinguished from Bama miniature pigs by using WM27, and from Huangjiang miniature pigs by using WM10. In conclusion, the ISSR method enables rapid and accurate identification of fresh