国产水溶性蜂胶对主要致龋链球菌及其耐氟菌株致龋性的影响彭志庆1.docVIP

国产水溶性蜂胶对主要致龋链球菌及其耐氟菌株致龋性的影响 彭志庆1，林居红1，刘明方2，邓一平2 （1重庆医科大学附属口腔医院预防科， 重庆 400015，2重庆医科大学实验教学中心， 重庆400016） 摘要： 目的 了解国产水溶性蜂胶对变形链球菌（S.mutans ATCC 25175，S.m）、远缘链球菌（S.sobrinus 6715，S.s)及其耐氟菌株的生长抑制作用，以及对葡糖基转移酶（GTF）的影响，探讨一种新的防龋途径。方法 采用最小抑菌浓度（MIC）递增法对S.m、S.s进行氟化钠体外诱导耐氟菌株（S.m-FR、S.s-FR），采用液体稀释法观察水溶性蜂胶对S.m、S.m-FR、S.s、S.s-FR生长的影响；用酶化学分析方法检测蜂胶对GTF酶活性的影响。结果 水溶性蜂胶对S.m、S.m-FR、S.s、S.s-FR最小抑菌浓度（MIC）分别为0.39、0.78、0.02、0.39g/L；最小杀菌浓度（MBC）分别为0.78、1.56、1.56、1.56g/L；随着蜂胶浓度增高，葡糖基转移酶活性逐渐降低，各组间差异具有统计学意义（P＜0.05）,各组与对照组比较差异有统计学（P＜0.05）Effect of propolis on the main cariogenic sreptococcus and their fluoride-resistant strains,their glucosyltransferase Peng Zhi-qing1, Lin Ju-hong1,,Liu Ming-fang2,Deng Yi-ping2 （1Department of Preventive Dentistry, Affiliated Stomatological Hospital, Chongqing Medical University，Chongqing, 400015；2 Experimental Teaching Center，Chongqing Medical University，Chongqing, 400016，China） Abstract Objective To evaluate the effects of propolis on the growth of S.mutans ATCC 25175(S.m),S.sobrinus 6715(S.s) and their fluoride-resistant strains(S.m-FR,S.s-FR),and the change of their glucosyltransferase(GTF). Methods S.m and S.s were induced with sodium fluoride by minimum inhibitory concentration(MIC) method for Fluoride-resistant strains(S.m-FR,S.s-FR).Fluid dilution method was used to observe the effects of different levels of propolis on growth of S.m、S.m-FR、S.s、S.s-FR. Using the method of chemistry enzyme analysis technique to detect the influence of propolis on the enzyme activity of GTF. Results MIC of propolis for S.m、S.m-FR、S.s、S.s-FR were respectively 0.39,0.78,0.02,0.39g/L . MBC of propolis for them were respectively 0.78,1.56,1.56,1.56g/L；Glucosyltransferase of S.m,S.m-FR,S.s,S.s-FR decreased gradually with the increase of each concentration of Propolis.There were significant differences among total sample groups,and each experimental group and positive control group as well (P＜0.05). Conclusion The use of propolis could effectively inhibit the growth of S.