霍山铁皮石斛多糖的脱蛋白工艺及结构分析-食品科学.pdfVIP

164 2017, Vol.38, No.12 食品科学 ※工艺技术 霍山铁皮石斛多糖的脱蛋白工艺及结构分析 * 王琳炜，欧阳臻 ，张碧娟，杨 倩，王笃军，于一凡，张 杰，马 青 （江苏大学药学院，江苏镇江 212013 ） 摘  要：优选霍山铁皮石斛多糖脱蛋白工艺，并对其进行分离纯化及结构分析。以蛋白脱除率和多糖损失率为评 价指标，比较Sevag法、酶法及酶-Sevag法3 种脱蛋白方法。结果表明，酶-Sevag法脱蛋白效果最佳，蛋白脱除率 为81.58%，多糖损失率为15.63% 。采用DEAE-52纤维素柱、Sephadex G-100凝胶柱分离纯化石斛多糖，得活性组 分DOPA-1 ；采用紫外扫描光谱、高效液相色谱鉴定DOPA-1为均一多糖；采用气相色谱分析表明组分单糖由甘露 糖、葡萄糖和半乳糖组成（各单糖的物质的量比为1∶0.42 ∶0.27 ）；采用红外光谱和刚果红实验分析糖链结构表明， β D DOPA-1有典型的糖类特征吸收峰，存在 - -甘露吡喃糖苷，且具有三股螺旋构象。 关键词：铁皮石斛多糖；脱蛋白；分离纯化；结构分析 Deproteinization and Structural Analysis of Polysaccharides from Dendrobium officinale Kimura et Migo Grown in Huoshan * WANG Linwei, OUYANG Zhen , ZHANG Bijuan, YANG Qian, WANG Dujun, YU Yifan, ZHANG Jie, MA Qing (School of Pharmacy, Jiangsu University, Zhenjiang 212013, China) Abstract: In this study, the deproteinization and structural characterization of the polysaccharides extracted from Dendrobium officinale Kimura et Migo grown in Huoshan county of Anhui province were investigated. Three protein removal methods: the Sevage method, enzymatic hydrolysis and their combination were comparatively evaluated in terms of protein removal and polysaccharide loss rates. The combined method was found to have the highest deproteinization efficiency, yielding 81.58% protein removal and 15.63% polysaccharide loss. After purification of the polysaccharides by successive DEAE-cellulose-52 and Sephadex G-100 column chromatography, an active fraction named as DOPA-1 was obtained. By using UV spectroscopy and high performance liquid chromatography (HPLC)