姜黄素对大鼠重症胰腺炎的干预效应研究-第三军医大学学报.docVIP

姜黄素对大鼠重症胰腺炎的干预效应研究 李晓萍1，张志坚400038重庆，第三军医大学西南医院急救部巴南区人民医院ICU(acute pancreatitis，SAP)发病过程中的作用及姜黄素（curcumin，CUR）的干预效应。方法 54只SD大鼠按完全随机法分为SAP组、姜黄素治疗(SAP+CUR)组和对照组(control)，用4%牛磺胆酸钠逆行胆胰管注射建立大鼠模型，SAP+CUR组在建立模型前2 h腹腔内注射姜黄素溶液（200 mg/kg），余组注射同等剂量的DMSO溶液ELISA法测定3组大鼠不同时点血清中ENA-78水平的变化； Western blot和免疫组化法检测胰腺组织不同时点ENA-78蛋白的表达水平；病理组织切片检查各时点胰腺的病理变化。结果 SAP组血清中ENA-78水平随时间的延长逐渐增强，3 h后各时点血清ENA-78水平高于对照组(PO.01)，6 h后SAP组与SAP+CUR组血清ENA-78水平差异显著(PO.05)；SAP组及SAP+CUR组术后胰腺组织ENA-78蛋白表达随时间延长逐渐增加，术后各时间点表达均显著高于对照组(P0.05或P0.01)，但同时间点比较SAP+CUR组表达明显低于SAP组(P0.05，P0.01)；病理组织切片检查显示SAP+CUR组各时点与SAP组相比较，胰腺病理损伤显著减轻。结论 姜黄素能有效干预SAP，其机制可能与抑制趋化因子ENA-78的表达有关。 [关键词] 重症急性胰腺炎；姜黄素；；LI Xiao-ping1，ZHANG Zhi-jian，Department of Emergency，Southwest Hospital，Third Military Medical University，Chongqing400038; 2Intensive Care Unit，Banan District People’s Hospital，Chongqing, 401320，China) [Abstract] Objective To investigate the role of epithelial neutrophil activating peptide 78(ENA-78) in the pathogenesis of severe acute pancreatitis(SAP) and effects of curcumin intervention on ENA-78 expression in SAP. Methods Fifty-four SD rats were randomized into three group:SAP group,curcumin treatment group(SAP+CUR) and control group.SAP model was induced by retrograde infusion of 4% sodium taurocholate into the bili-pancreatic duct in the rats.Treated group was injected curcumin (200 mg/kg) through abdominal cavity before operation while control and SAP groups were injected nolal-dimethyl sulfoxide.ENA-78 in serum was detected by ELISA at various time points.The expression of ENA-78 in pancreatic tissues was detected by Westen blotting and immunohistochemistry,the histopathological changes were observed at the same time.Results Serum level of ENA-78 3 h after operation in SAP group was significantly higher than that in control group(P0.05). Serum level of ENA-78 6 h after operation in SAP group was significantly higher than that in SAP+CUR group (P0.05). Compared with contral group,the expression of ENA-78 in the pancre