银合欢种子多糖微结构的原子力显微镜观察.pdfVIP

※基础研究 食品科学 2012, Vol. 33, No. 15 99 1 1 2 2 3 1 杨永利 ，郭守军 ，叶文斌 ，潘显辉 ，林月娟 ，林海雄 (1.韩山师范学院生物系，广东 潮州 521021 ；2.西北师范大学生命科学学院，甘肃 兰州 730070 ； 3.汕头大学分析测试中心，广东 汕头 515063) 目的：通过对银合欢种子多糖的形貌观察，研究其微结构。方法：采用热水浸提法提取银合欢种子多糖， 通过改变溶液的质量浓度、云母基底表面的化学修饰等不同制样方式，用原子力显微镜(AFM)可视化不同样品制备 条件形成的聚集体和非聚集体银合欢半乳甘露聚糖结构形貌。结果：样品在不同制备条件下得到膜状、颗粒状的 聚集体结构和非聚集体单糖分子结构。结论：银合欢种子多糖的单个糖分子呈线形螺旋状，并具有短的分枝结构， 分子链间互相缠绕，而形成网格状。 原子力显微镜； 银合欢多糖； 微结构 Microstructural Observation of Leucaena glaucal Seed Polysaccharides under Atomic Force Microscope 1 1 2 2 3 1 YANG Yong-li ， GUO Shou-jun ， YE Wen-bin ，PAN Xian-hui ，LIN Yue-juan ，LIN Hai-xiong (1. Department of Biology, Hanshan Normal University, Chaozhou 521021, China ；2. College of Life Science, Northwest Normal University, Lanzhou 730070, China ；3. Analysis and Testing Center, Shantou University, Shantou 515063, China) Abstract ：Objective: The microstructure of Leucaena glaucal seed polysaccharides was studied based on morphological observation under atomic force microscope (AFM). Methods: Polysaccharides from were extracted by hot water extraction. The structure and morphology of aggregates and non-aggregates from Leucaena glaucal seed polysaccharides formed through varying polysaccharide concentration or chemically modifying the surface of mica substrates were visualized under AFM. Results: Membranous, granular aggregates structure and non-aggregate structure of a single polysaccharide molecule could be observed in the samples prepared at various conditions. Conclusion: A single Leucaena glaucal seeds polysaccharide molecule was linear spiral