分光光度法测定大豆卵磷脂中磷脂酰胆碱含量-食品安全质量检测学报.PDFVIP

第4 卷 第2 期 食品安全质量检测学报 Vol. 4 No. 2 2013 年4 月 Journal of Food Safety and Quality Apr. , 2013 刘鹏莉, 张双灵, 魏玉良, 庄桂东, 于 丹, 迟玉森* (青岛农业大学食品科学与工程学院, 青岛 266109) 摘 要: 目的 建立一种简单、准确的卵磷脂测定方法。方法 采用KOH 溶液将磷脂酰胆碱中的胆碱游离, 加 雷氏盐反应后, 在波长525 nm 处, 通过分光光度法测定胆碱的量。结果 该方法在0.075 ～0.225 mmol 范围内 线性关系良好, 线性方程为 Y = 3.6457X+0.026, R2 = 0.9987, 方法的回收率在95% ～103%之间, 相对标准偏差 小于 1.0%, 待测液在2.5 h 之内稳定, 能够满足检测要求。结论 本方法具有操作简单、准确性高、稳定性好、 抗干扰性强的优点, 可以用于卵磷脂产品中磷脂酰胆碱含量的测定。 关键词: 卵磷脂; 磷脂酰胆碱; 碱水解; 分光光度法; 雷氏盐 Determination of phosphatidylcholine in soybean lecithin by spectrophotometry * LIU Peng-Li, ZHANG Shuang-Ling, WEI Yu-Liang, ZHUANG Gui-Dong, YU Dan, CHI Yu-Sen (College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China) ABSTRACT: Objective To establish a simple and accurate method for determination of phosphatidylcho- line. Methods Free choline was obtained from phosphatidylcholine by alkaline hydrolysis and was deter- mined by spectrohpotometry under the wavelength of 525 nm after reaction with Reinecke’s salt. Results The method showed a good linearity over the range of 0.075m mol ～0.225m mol,and the linear equation was Y = 3.6457X +0.026 with R2=0.9987. The recoveries was in range of 95% ～103%, and the relative standard devia- tions (RSDs) were lower than 1%. The liquid was stable in 2.5h, which can meet the test