基因重组心肌肌钙蛋白I融合蛋白抗肿瘤作用实验研究.pdfVIP

World Journal of Cancer Research 世界肿瘤研究, 2017, 7(2), 48-55 Published Online April 2017 in Hans. /journal/wjcr /10.12677/wjcr.2017.72008 The Experimental Study of Tumor Growth of Recombinant Protein Fused with Cardiac Troponin I and Artificial Peptide 1* 2 2 1 1 2 2* Zhaoyang Liu , Jie Tian , Fenghua Shi , Jun Liu , Xiao Liang , Muxiu Tan , Fengming Liu 1National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 2 Guangxi Medical Botanical Institute, Nanning Guangxi st th th Received: Apr. 1 , 2017; accepted: Apr. 15 , 2017; published: Apr. 20 , 2017 Abstract Objective: The effect of antitumor growth of recombinant cardiac troponin fusion protein com- posed of subunit I and artificial peptide, called CIS, was examined in this study. Methods: The CIS’s effect on the expressing of human vascular endothelial growth factor A (VEGF-A) was examined using Western blot assay in vitro, and matrix metallopeptidase 9 was studied with purified recom- binant CIS protein. The effect of tumors growth treated with CIS was performed using several in vivo nude mice xenograft models. Results: There was an evident reduction on VEGF-A, MMP-9 when the human hepatocellular carcinoma cells MHCC97H were treated with purified CIS fusion protein, which was also shown in dose-dependent manner compared to the untreated control group. A significant inhibition of tumor growth rate was achieved in CIS treated nude mice com- pared to untreated control mice in 8 or 6 different nude mice xenograft mo