磁修饰酵母对直接大红染料的生物吸附-微生物学报.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2009, October 25; 25(10): 1477-1482 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2009 Institute of Microbiology, CAS CSM, All rights reserved 环境生物技术 吴琦, 单志, 沈茂, 李双江, 陈惠 , 625014 : 本研究采用水相合成的纳米级铁磁流体对灭活酿酒酵母细胞进行磁修饰, 获得了具有良好磁响应的酵母。傅立 叶变换红外光谱分析表明, 该修饰酵母在Fe-O 特征峰581 cm−1 处的吸收明显加强。透射电镜观察表明, 纳米磁性颗粒 单个或成团聚集在酵母细胞表面。在本实验条件下, 160 μL 磁修饰酵母对 1 mL 浓度为0.4 mg/mL 直接大红染料吸附率 达 100%; 8 min 达到吸附平衡; 在70% 乙醇中, 染料脱吸附率为99.18% 。由于磁修饰酵母吸附力强, 吸附速度快, 易于 磁分离, 是一种有前景的水溶性染料生物吸附剂。 : 酿酒酵母, 磁修饰, 直接大红, 生物吸附 Biosorption of direct scarlet dye on magnetically modified Saccharomyces cerevisiae cells Qi Wu, Zhi Shan, Mao Shen, Shuangjiang Li, and Hui Chen College of Life Science, Sichuan Agricultural University, Ya’an 625014, China Abstract: We prepared the nano-sized magnetic fluid in aqueous phase solution to modify the killed yeast cells (Saccharomyces cerevisiae). Subsequently, the yeast cells with good magnetic response was obtained.The infrared spectroscopy analysis showed that the modified yeast cells increased obviously the absorption at the Fe-O characteristic peak of 581 cm−1. Moreover, the transmission electron microscopy picture indicated the magnetic nanoparticles presented individually or aggregately on the yeast cell surface. Under the experimental conditions, the magnetically labeled yeast with 160 μL showed the adsorption percentage up to 100% in 1 mL direct scarlet dye solution with the concentration of 0.4 mg/mL, and arrived at the adsorption equilibrium within 8 min. Besides, the adsorbed dyes can b