油茶饼粕中黄酮苷类化合物的分离与结构鉴定.pdfVIP

3 1 1 V o.l 3 1 N o. 1 20 11 2 Ch em istry and Industry of Forest P rodu cts Feb. 2011 * 陈虹霞, 王成章, 叶建中, 周 昊 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实 室; 国家林业局林产化学工程重点开放性实 室, 江苏 南京 2 10042) : 以茶籽饼粕为原料, 采用体积分数 65% 乙醇- 水溶液提取, 并通过中低压色谱快速分 离得到高纯度的黄酮苷混合物进 一步采用高效液相色谱制备, 柱子 XB- C 18 10mm @ CHEN H ong-x ia 250 mm, 5 Lm ) , 流动相甲醇-水 体积比 2B3) , 紫外 270 nm, 流速 3 mL /m in, 分离得到黄酮苷化 合物Ñ 和Ò 经 IRM S和 NM R 鉴定, 两种化合物分别为山奈酚 3- O- [ 2- O- B-D- 半乳糖- 6- O- A-L- 鼠李糖] - B-D-葡 萄糖苷 Ñ ) 和山奈酚 3- O- [ 2- O- B-D-木糖- 6- O- A-L-鼠李糖] - B-D-葡萄糖苷 Ò ) : 油茶饼粕; 黄酮苷; 高效液相色谱; 分离 : TQ351 : A : 0253- 2417 2011) 01- 00 13- 04 Isolation and Identification of F lavono id G lycosides from Gam ellia olefi era A be.l Cake CH EN H ong-x ia, WANG Cheng-zhang, YE J ian-zhong, ZHOU H ao Institute o f Chem ica l Indu stry o f F orest P roducts, CA F; N ational Eng inee ring L ab. fo r B iom ass Chem ica l U tiliza tion; K ey and O pen L ab. on Forest Chem ica l Eng ineer ing, SFA, N an jing 210042, China) Abstract: The dreg-cakes of o il came llia Cam ellia d eif era A bel. ) w ere ex tracted w ith 65% e thano l. The ex trac t w as separated by m iddle-pressure chrom atography. The flavoniod g lycosides m ixture in high purity w as obta ined tw o com pounds w ere iso lated by HPLC, u sing XB-C 18 co lum n 10 mm @ 250 mm, 5 Lm ) w ith a mob ile phase w hich consists of a m ixture o