甜瓜种质资源遗传多样性的srap分析geneticdiversityofa.pdfVIP

HEREDITAS (Beijing) ISSN 0253-9772 甜瓜种质资源遗传多样性的 SRAP 分析 1，2 1 1 陈芸 ，李冠 ，王贤磊 1.新疆大学生命科学与技术学院，乌鲁木齐 830046 ； 2.喀什师范学院生命与环境科学系，新疆喀什 844000 摘要：为研究甜瓜材料之间的亲缘关系及其分类，更有效地利用种质资源，为培育新品种提供依据，文章采用SRAP (Sequence-related amplified polymorphism)技术对61份甜瓜种质资源的遗传多样性进行研究。结果表明：从42对引物组合中筛 选出16对扩增条带清晰、多态性高的引物组合分析供试材料，共检测出452个位点，其中265个为多态性位点，多态性比率达 58.63 ％，平均每对引物组合产生28.56个位点和16.56个多态性位点。61份材料间的相似系数为0.48～0.93，平均为0.73 。这些 结果说明，供试甜瓜材料具有较为丰富的遗传多样性。聚类分析结果表明，61个甜瓜品种中首先可分为薄皮甜瓜与厚皮甜瓜 两大类，彼此亲缘关系最远。以遗传相似系数0.74为截值，可把供试材料分为5个类群。在生态区域中，新疆厚皮甜瓜的Nei’s 基因多样性指数(0.2231)和Shannons信息指数(0.3422)最高。 关键词：甜瓜；SRAP；遗传多样性 Genetic diversity of a germplasm collection of Cucumis melo L. using SRAP markers 1, 2 1 1 CHEN Yun , LI Guan , WANG Xian-Lei 1.College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; 2. Department of Life and Environmental Science, Kashgar Teachers College, Kashgar 844000, China Abstract: Genetic relationships and classifications of a set of melon accessions were analyzed to provide the experimental support for utilizing effectively genetic materials for breeding. Sequence-related amplifled polymorphism technique was adopted to analyze 61 melon accessions. Sixteen primer combinations with clear band pattern and polymorphism were selected from over 42 primer combinations. Four hundred and fifty-two loci were detected by 16 pairs of SRAP primers.Among them,265 were polymorphic,the polymorphic rate was 58.63%,and 28.56 loci and 16.56 polymorphic loci were amplified by each pairs of primers on average. The genetic similarity coefficient of the 61 accessions ranged from 0.48 to 0.93,with an average of 0.73. These results suggested that there was rich genetic diversity among the melon accessions tested. The varieties examined were clustered into two groups,which were thick-skinned melon and thin-skinned melon.Five groups were clustered according to genetic similar