甜蛋白brazzein基因在番茄果实中的特异表达-遗传.pdfVIP

HEREDITAS (Beijing) 2009 6 , 31(6): 663―667 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00663 甜蛋白Brazzein 基因在番茄果实中的特异表达 1 2 1 3 1, 4 尹涛 , 卢虹玉 , 张上隆, 刘敬梅 , 陈大明 1. , 310029; 2. , 524088; 3. , 100089; 4. Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, USA 西瓜(Citrullus vulgaris S.)来源的AGPL1 启动子在番茄(Lycopersicon esculentum L.)果实中具有较强的特 异性驱动功能。将该启动子与甜味蛋白基因 Brazzein 融合构建植物表达载体, 通过根癌农杆菌(Agrobacterium tumefaciens)介导法成功地进行了对番茄的遗传转化, 获得转化植株。组织化学法、PCR 特异扩增、Southern 杂 交分析及RT-PCR 检测, 表明Brazzein 基因已整合到转基因番茄植株基因组中并且稳定表达。通过AGPL1 果 实特异启动子的调控, 在不改变果实其他性状的前提下提高了番茄果实甜味品质, 并为甜蛋白的生产提供 经验。 番茄; 甜蛋白基因Brazzein ; 遗传转化; 果实特异; AGPL1 启动子 Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants 1 2 1 3 1, 4 YIN Tao , LU Hong-Yu , ZHANG Shang-Long , LIU Jing-Mei , CHEN Da-Ming 1. Department of Horticulture , Zhejiang University, Hangzhou 310029, China; 2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 3. Beijing Vegetable Research Center, Beijing 100089, China; 4. Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, USA Abstract: The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vec- tor harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium -mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis a