柚类种质资源aflp与ssr遗传多样性分析-中国农业科学.pdfVIP

中国农业科学 2005,38(11):2308-2315 Scientia Agricultura Sinica 柚类种质资源AFLP与SSR遗传多样性分析 1,2 1 2 2 1 刘 勇 ，孙中海 ，刘德春 ，吴 波 ，陶建军 1 2 （华中农业大学柑桔研究所，武汉 430070 ； 江西农业大学农学院，南昌 330045) 摘要：结合 SSR 引物和 AFLP 分子标记对 110 份柚类基因型、12 份野生近缘种进行遗传多样性研究。结果表 明，SSR标记的335个位点中99.1%为多态性位点，每个位点可检测到9.85个等位基因，基因多样度 （GD）变幅 为0.1939～0.9073，获得了46个特异性SSR 标记；AFLP标记的343个位点中72%为多态性位点，3对引物平均期 望杂合度变幅为0.21863～0.28445,平均每对引物组合能产生82个多态位点，获得了44个AFLP特异性标记。UPGMA 聚类结果显示，122份基因型在相似系数0.61时，分成8个组群，其中柚类主要由沙田柚品种群、文旦品种群与 庞大的杂种柚品种群组成。分类结果将有利于更好地利用这些丰富的育种资源。 关键词：柚类；种质资源；遗传多样性；SSR；AFLP Assessment of the Genetic Diversity of Pummelo Germplasms Using AFLP and SSR Markers 1, 2 1 2 2 1 LIU Yong , SUN Zhong-hai , LIU De-chun , WU Bo , TAO Jian-jun 1 2 ( Citrtus Research Institute, Huazhong Agricultural University, Wuhan 430070; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045 ) Abstract: Genetic diversity of 110 pummelo germplasm and 12 its relatives were analyzed by SSR and AFLP. Approximately 99.1% of the 335 SSR were polymorphic, and 9.85 alleles per SSR loci were identified. Gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 accessions of pummelo and their relatives were divided into eight groups, in which