氯化锂-匹罗卡品致痫幼鼠成年后脑内脱髓鞘变化-第三军医大学学报.docVIP

幼鼠癫痫持续状态成年后脑内髓鞘变化的研究 刘淑丹，王晗知，何扬涛，肖岚，李红丽 （400038 重庆第三军医大学基础医学部组织学与胚胎学教研室，发育生物学教研室） 摘要 目的 探讨致幼鼠癫痫持续状态（status epilepticus, SE）成年后发展为自发反复性癫痫发作( spontaneous recurrent epileptic seizure, SRS)的大鼠脑内髓鞘的变化。 方法 采用氯化锂-匹罗卡品腹腔注射诱导幼鼠SE模型，应用多道生理信号采集处理系统检测大鼠脑电图（EEG）异常活动波，采用Nissl染色方法观察脑内神经元的变化，证实SRS模型大鼠诱导成功；应用卢卡斯快蓝（uxol fast blue , LFB）染色及髓鞘碱性蛋白(myelin basic protein ,MBP)免疫组织化学染色等技术观察脑内髓鞘的变化。结果 SRS模型组脑电图与正常对照组相比，可见频发尖波、棘-慢、尖-慢综合波；海马CA1、CA3和齿状回门区Nissl染色显示神经元数目减少，部分细胞变性和坏死。SRS模型组在胼胝体(cc)与扣带回(cg)区域内的LFB染色可见着色范围明显缩小，着色浅淡，与正常对照组相比有显著差异；在胼胝体(cc)、扣带回（cg）、第一躯体感觉皮质区（S1BF）和第一听皮质区（Au1）域内MBP免疫阳性沉淀的平均光密度值（OD）显著低于正常对照组（0.05）。结论 致痫幼鼠成年后伴有慢性癫痫发作可造成CNS 髓鞘脱失。 关键词氯化锂-匹罗卡品慢性癫痫脑电图脱髓鞘髓鞘碱性蛋白 Study on Changes of myelin of rats induced status epilepticus in juvenile stage Liu Shudan, Wang Hanzhi, He Yangtao, Xiao Lan, Li Hongli（Department of Histology and Embryology, Department of Developmental Biology, Institute of Neuroscience, The Third Military Medical University, Chongqing 400038, China）Abstract] Objective To investigate the change of myelin in the brain of the mature rats with spontaneous recurrent epileptic seizure(SRS) after induced status epilepticus(SE) in immature. Methods The model of juvenil SE rats was induced by lithium–pilocarpine administration. Electroencephalogram (EEG) was used to monitor the abnormal wave of epilepsy; Nissl staining was used to observe the alteration of neuron in hippocampus. Then Luxol Fast blue (LFB) staining and the expressions of myelin basic protein (MBP) were studied by histopathological and immunohistochemistry methods. Results The EEG and Nissl staining confirmed the rats were induced successfully to SRS model ; the positive LFB staining of the model group turned to lighter and smaller especially in the callosum corpus(cc）, cingulated gyrus(cg)and white matter of hippocampus. The average optical density（OD）of MBP positive area in the callosum corpus(cc）, cingulated gyrus(cg), primary somatosensory cortex,barrel field (S1BF) and primary auditory