冰乙酸水解法提高女贞子提取物中红景天苷含量-应用与环境生物学报.PDFVIP

2013-08-25 DOI: 10.3724/SP.J.1145.2013.00723 / Chin J Appl Environ Biol 2013 19 ( 4 ) : 723-726 应用与环境生物学报 ， 冰乙酸水解法提高女贞子提取物中红景天苷含量* 1 1, 2 1** 郭加加 黄君梅 王飞 1 （中国科学院成都生物研究所 成都 610041） 2 （成都中医药大学 成都 610075） 摘 要 为了提高女贞子提取物中红景天苷的含量，以红景天苷和酪醇的含量为考察指标，采用高效液相色谱法测定 其含量，利用正交试验设计优选女贞子提取物中红景天苷的水解工艺，并考察料液比、水解时间和水解温度对提取 物中红景天苷含量的影响. 结果发现其中冰乙酸与提取液的料液比以及水解时间对提取物中红景天苷的含量起着主 20 1 100 20 h. 要作用；最佳工艺条件为：女贞子浓缩液与冰乙酸体积比为 ︰， ℃下回流 水解后红景天苷的含量约是未 经酸水解的3倍. 研究表明冰乙酸水解法可显著提高女贞子提取物中红景天苷的含量；该方法操作简单，后续处理容 易，水解原料无毒，可以满足大规模工业提取的需要. 图1 表3 参 15 关键词 酸水解法；女贞子；红景天苷；正交试验 CLC Q946.83 : Q949.776.2 Increasing Salidroside Content from the Extract of Ligustrum lucidum Ait. Fruits by Acid Hydrolysis* 1 1, 2 1** GUO Jiajia , HUANG Junmei WANG Fei 1 ( Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) 2 ( Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China) Abstract To develop an acid hydrolysis method to increase salidroside content from the extract of Ligustrum lucidum Ait. fruits, this study, using salidroside and tyrosol contents as indexes, adopted the thogonal design to acquire the optimum conditions for the extraction of salidroside by varying the feed liquid ratio, hydrolysis time and hydrolysis temperature. The contents of both salidroside and tyrosol were measured by HPLC. We found that the feed liquid ratio and hydrolysis tim