胶体金技术的应用及展望.ppt

;目录;胶体金的概念;特征性质;光吸收性胶体金在可见光范围内有一单一光吸收峰，这个光吸收峰的波长（λ）在510～550nm范围内，随胶体金颗粒大小而变化，大颗粒胶体金的λmax偏向长波长，反之，小颗粒胶体金的λmin则偏于短波长;胶体金 - 制备方法与贮存;比较采用4种不同化学还原法制备出单分散性好的胶体金样品;免疫胶体金技术的应用;Colloidal gold based electrochemical immunoassays for the diagnosis of acute myocardial infarction（基于胶体金的电化学免疫诊断为急性心肌梗死）The performed immunoassays（免疫测定） are directed to the determination of protein markers for acute myocardial infarction（急性心肌梗死）. The determination assays for human myoglobin（肌红蛋白）, cardiac troponin complex（肌钙蛋白复合物） and the MB isoform of the enzyme creatine kinase are presented. Cyclic voltammetry measurements are adopted for the determination of colloidal gold used as a label in the immunuassays. The measurements are performed with a screen-printed graphite electrode in a sample volume of 50μl. The influence of different diameter colloidal gold particles on the assay sensitivity is also investigated.;Colloidal gold nanoparticle modified carbon paste interface for studies of tumor cell adhesion and viability（胶体金纳米改性碳对肿瘤细胞粘附和可行性研究的粘贴界面） A non-toxic biomimetic interface for immobilization of living cells and electrochemical exogenous effect study of cell viability was constructed by mixing colloidal gold nanoparticles in carbon paste. A new approach to study the effects of anti-tumor drug and other exogenous factors on cell viability was proposed. The nanoparticles were efficient for preserving the activity of immobilized living cells and preventing their leakage from the electrode surface. The immobilized living AsPC-1 cells (pancreatic adenocarcinoma cells derived from ascites) exhibited an irreversible voltammetric response related to the oxidation of guanine. The presence of guanine was verified by liquid chromatography–mass spectrometry. The contents of guanine in cytoplasm of each AsPC-1 and normal pancreatic cell were detected to be 370 and 22amol, respectively. The cytotoxic effect of adriamycin resulted in a decrease in peak current of guanine. The optimal exogenous factors