基因突变库的筛选方法.pptVIP

枯草杆菌蛋白酶E热稳定性的分子进化（Huimin Zhao等，1999） 进化后的枯草杆菌蛋白酶E 正面 反面 耐热p-硝基苯酯酶的分子进化(Lori Giver等，1998） β-葡糖苷酶耐热性的提高 (Mary′a Jesu′ s Arrizubieta等，2000） Evolution of a cytokine using DNA family shuffling Chia-Chun J. Chang ……. Phillip Patten Nature Biotechnology Vol. 17, August, 1999 Aims of the work: Demonstration of usefulness of the DNA shuffling tool for rapidly evolving human a interferon towards improving antiviral property Interferons (IFNa, IFNb, IFNg) Reasons for their limited use as drugs: IFNas are produced by monocyte, macrophages and other cells upon stimulation by virus.