松辽盆地十屋断陷流体一岩石相互作用-地球信息科学学报.pdfVIP

NEURAL REGENERATION RESEARCH April 2014,Volume 9,Issue 8 Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury 1 1 1 1 1 2 Zhiyuan Li , Zhanxiu Zhang , Lili Zhao , Hui Li , Suxia Wang , Yong Shen 1 Department of Joint Orthopedics, Hebei Provincial Xingtai People’s Hospital, Xingtai, Hebei Province, China 2 Department of Spinal Orthopedics, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China Abstract Corresponding author: We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone Zhiyuan Li, Department of Joint marrow mesenchymal stem cells before transplantation might further improve neurological Orthopedics, Hebei Provincial Xingtai function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and People’s Hospital, Xingtai, Hebei Province, BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone China, myarcher@126.com. marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was significantly enhanced in the model group. Af- doi: 10.4103/1673-5374.131595 ter 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo- / 66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated ner Accepted: 2014-02-08 ve fibers were detectable in the central t