云南芒果种质基因组大小测定与变异分析-热带亚热带植物学报.pdfVIP

热带亚热带植物学报　2015 ，23(4) ：386 ~ 390 Journal of Tropical and Subtropical Botany 云南芒果种质基因组大小测定与变异分析 柳觐, 李开雄, 孔广红, 倪书邦 (云南省热带作物科学研究所，云南 景洪 666100) 摘要： 为了解云南芒果(Mangifera indica L.)种质资源的基 因组 的变异情况 ，采用流式细胞术对 35 份云南芒果种质资源的基 因 组大小进行 了测定和变异分析。结果表明，云南芒果种质资源的基 因组大小存在一定差异 ，基 因组 的平均 C 值是 0.445110 pg ， 9 9 0.4353177×10 bp, 最小的是采 自景洪的半栽培种 YSM-44 (0.434567 pg, 0.4250060×10 bp) ，最大的是采 自红河 的野生种 YSM- 9 25 (0.458679 pg, 0.4485881×10 bp) 。基 因组 C 值变异程度最大的是野生种(CV = 1.65%) ，其次为半野生种(CV = 1.26%) 、半栽培 种(CV = 1.2 1%)和栽培种(CV = 0.11%) 。与芒果具有相近基 因组大小 的多为苔藓植物 ，与“ 值悖论”观点相一致 。因此 ，应用流 C 式细胞术能准确 、快捷地测定芒果基 因组大小 ，而且云南野生、半野生及半栽培芒果种质资源遗传变异类型丰富 ，有较大的挖 掘利用潜力 。 关键词： 芒果 ；种质资源 ；基 因组 ；变异 doi: 10.11926/j .issn.1005–3395.2015.04.004 Genome Size and Variation Analysis of Mango (Mangifera indica L.) Germplasms in Yunnan by Flow Cytometry LIU Jin, LI Kai-xiong, KONG Guang-hong, NI Shu-bang (Yunnan Institute of Tropical Crops, Jinghong 666100, China) Abstract: In order to understand the variation of mango (Mangifera indica L.) germplasms in Yunnan, the genome size of 35 germplasms was determined by flow cytometry and their variation was analyzed. The results showed that the mean genome size among the 35 germplasms was 0.445110 pg and 0.4353177×109 bp, which the minimum one (0.434567 pg, 0.4250060×109 bp) was YSM-44 from Jinghong, and the maximum one (0.458679 pg, 0109 bp) was YSM-25 from Honghe. The genome size variation of wild germplasms was the largest (CV=1.65%), followed by semi-wild germplasms (CV=1.26%), semi-cultivated germplasms (CV=1.21%) and cultivated germplasms (CV=0.11%). The bryophytes had similar genome size to mango, which is consistent with the “C-value paradox” theory. Therefore, flow cytometry method could accurately and fastly measure genome size of mango,