半制备高压液相色谱法制备罗汉果苷ⅴ标准品.pdfVIP

※工艺技术 食品科学 2010, Vol. 31, No. 12 137 1 1 1 2 宁德生 ，梁小燕 ，方 宏 ，姚崇辉 (1.广西壮族自治区- 中国科学院广西植物研究所，广西桂林 541006 ； 2.贺州市产品质量监督检验所，广西贺州 542800) ：目的：通过半制备高压液相色谱分离技术，建立一种快速、稳定、高效制备高纯度罗汉果苷Ⅴ标准品 的方法。方法：先采用树脂吸附脱色技术进行前处理，再通过半制备高压液相色谱分离制备罗汉果苷Ⅴ，色谱条 件为Eclipse XDB-C18 色谱柱(9.4mm ×250mm ，5 μm) ，流动相为乙腈- 水，采用梯度洗脱，流速4mL/min ，检 测波长210nm ，进样量36mg ，柱温38 ℃。结果：分离得到的罗汉果苷Ⅴ的纯度为99.1% 。结论：本方法具有 操作简单、快速、稳定和产品纯度高等优点，可应用于实际样品的检测。 ：罗汉果；罗汉果苷Ⅴ；半制备高压液相色谱 Preparation of Mogroside Ⅴ from the Fruits of Siraitia grosvenorii by Semi-Preparative HPLC 1 1 1 2 NING De-sheng ，LIANG Xiao-yan ，FANG Hong ，YAO Chong-hui (1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Science, Guilin 541006, China； 2. Hezhou Institute of Products Quality Supervision and Inspection, Hezhou 542800, China) Abstract ：Objective: Semi-preparative HPLC was employed to establish a fast, stable and efficient method for preparation of high purity mogroside V. Methods: The crude mogroside V extract from the fruits of Siraitia grosvenorii was decolorized by D101 macroporous resin column chromatography before semi-preparative HPLC separation. Based on the use of Eclipse XDB- C18 (9.4mm × 250 mm, 5μm) held at 38 ℃, the chromatographic conditions employed were as follows: mobile phase, a mixture of acetonitrile and water at a flow rate of 4 mL/min in the gradient elution mode, detection wavelength, 210 nm; abd sample load 36 mg. Results: The purity of the product obtained was as high as 99.1%. Conclusion: The developed method, thanks to the benefits of simplicity, rapidity, good stability and obtaining of high purity prod