大鼠白细胞介素6il-6elisakit.pdfVIP

武汉基因美科技有限公司 Ratinterleukin6(IL-6) ELISA Kit CatalogueNumber:JYM0646Ra  Forresearchuseonly.  For the quantitative in vitro determination of IL-6 concentrations in Rat serum, plasma, culture media or any biological fluid.  Expirationdate：sixmonths.  Storage： 2-8℃. Principle ThisELISA kit uses Sandwich-ELISA asthemethod.TheMicroelisa stripplateprovided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then aHorseradish Peroxidase (HRP)- conjugated antibody specific for IL-6 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-6 and HRP conjugated IL-6 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-6. You can calculate the concentration of IL-6 in the samplesby comparing the OD ofthe samples to the standard curve. 地址：武汉市洪山区高新大道666号光谷生物城 电话：027 1 网址： E-mail:jiyinmei027@163.com 武汉基因美科技有限公司 Materialsprovidedwiththekit Materialsprovidedwith 48determinations 96determinations Storage thekit 1 User manual 1 1 R.T. Closureplate 2 2 2 R.T. membrane 3 Sealedbags 1 1 R.T.