牛奶过敏原β-乳球蛋白的单克隆抗体制备及其双抗体夹心法的建立.pdfVIP

6 11 Vol. 6 No. 11 2015 11 Journal of Food Safety and Quality Nov. , 2015 β- 陈献雄, 邬玉兰, 吉琼梅, 杨平常, 刘志刚* ( , 518060) 摘 要: 目的 β-(β-LG), 方法 β-LG BALB/c , NS-1 , A Ig Ig ELISA Western Blot , β-LG结果 β-LG 6 , 1H8, 4A7, 4C3, 1F9, 1G5, 3D11, 20 , 6 IgG1 Western Blot 6 β-LG, 6 , 1G5 3D11 ELISA , β-LG : 15.625 ng/mL, 15.625~250 ng/mL 结论 6 , β-LG , 关键词: β-lactoglobulin ; ; ; Preparation of monoclonal antibodies and establishment of a sand- wich-ELISA system against allergen β-lactoglobulin from milk * CHEN Xian-Xiong, WU Yu-Lan, JI Qiong-Mei, YANG Ping-Chang, LIU Zhi-Gang (Institute of Allergy and Immunology , Shenzhen University, Shenzhen 518060, China) ABSTRACT: Objective To prepare and characterize monoclonal antibodies against allergen β-lactoglobulin (β-LG) in milk and establish a method for the detection of milk allergens by sandwich-ELISA . Methods BALB/c mice were immunized with β-LG-the main allergen from milk. The fusion cell strain secreting antibo- dies stably was screened by using hybridoma and limit dilution technique. The cells used to induce ascites in mice and monoclonal antibodies were purified by affinity chromatography on immobilized protein A. The sub- 基金项目: (2013158925) (2013B051000088)(GJHZ20130408174112021) (JCYJ20140418095735604) Fund: Supported by the Natio