不同嗜酸氧化亚铁硫杆菌DY15DY26DC对黄-中国有色金属学报.PDF

21 4 2011 4 Vol.21 No.4 The Chinese Journal of Nonferrous Metals Apr. 2011 1004-0609(2011)04-0932-07 (DY15, DY26, DC) Afe0022 1, 2 1, 2 1, 2 1, 2 1, 2 1, 2 (1. 410083 2. 410083) 3 Acidithiobacillus ferrooxidans (At . ferrooxidans)DY15DY26 DC Cu2+ Cu2+ Real time RT(Reverse transcriptase) PCR Afe0022 Cu2+ DY15DY26 DC Cu2+0.400.22 0.04 mol/L 9K 3 DY15DY26 DC pH 7 d 1.8 0.81.2 1.3 DY15 3 40 h DY15DY26 DC Fe2+ 80%100%100%DY15 DY26 DC DY15 3 Cu2+Afe0022 Cu2+At. ferrooxidans Cu2+ Q819 A Bioleaching of chalcopyrite by Acidithiobacillus ferrooxidans DY15, DY26 and DC and difference expressions of gene Afe0022 WU Xue-ling1, 2, YUAN Peng1, 2, HU Qi1, 2, HOU Dong-mei1, 2, MIAO Bo1, 2, QIU Guan-zhou1, 2 (1. School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China) Abstract: The effects of different initial concentrations of Cu2+ on chalcopyrite concentrate bioleaching and the difference of ability in response to copper ion (Cu2+) were investigated with three strains of Acidithiobacillus ferrooxidans (At . ferrooxidans) DY15, DY26 and DC. The real time reverse transcriptase (RT) PCR was used to analyze the difference expression of gene Afe0022 cation channel protein in copper ion with different concentrations. The results show that