基因芯片的生物信息学课件.pptVIP

Microarray and Bioinformatics基因芯片的生物信息学 Dr Jingfu Qiu 邱景富 School of Public Health 公共卫生学院 Aims for the Microarray Bioinformatics Understand basic microarray technology and its use in gene expression analysis. 基因芯片技术与表达谱分析中的应用 Learn basic data analysis methods and how to apply them in the analysis of gene expression data 基因芯片的数据分析 Data acquisition 数据获得 Data normalization 数据归一化 Data analysis 数据分析 Data Clustering 数据聚类 Vocabulary-Review 回顾 Gene 基因: hereditary DNA sequence at a specific location on chromosome. Genetics 遗传学: study of heredity variation in organisms. Genome 基因组: an organs total content (full DNA sequence) Genomics 基因组学: study of organisms in terms of their genome. 2002年2月12日, 历时10载耗资20亿美元的人类基因组计划最终完成, 并报道了99% 的人类基因组序列. Vocabulary-Review回顾 Protein 蛋白质 : sequence of amino acids that “does something” Proteomics 蛋白质组学 : study of all of the proteins that can come from an organisms genome Bioinformatics 生物信息学 : the collection, organization analysis of large-scale, complex biological data. Functional Genomics 功能基因组学: study of obtaining an overall picture of genome functions, including the expression profiles at the mRNA level and the protein level Microarray 基因芯片 – A high throughput technology that allows detection of thousands of genes Simultaneously – gene chip, biochip ,array – Much rely on computer aids – Central platform for functional genomics Types of Microarrays 芯片的种类 DNA microarrays, such as cDNA microarrays and oligonucleotide microarrays MMChips, for surveillance of microRNA populations Protein microarrays Tissue microarrays Cellular microarrays (also called transfection microarrays Chemical compound microarrays Antibody microarrays Types of DNA Microarrays 1. cDNA chip (DNA microarray, two-channel array) cDNA芯片 : – Probe cDNA (500~5,000 bases long) is immobilized to a solid surface such as glass – Using robot spotting – Traditionally called DNA microarray – Firstly developed at S