不同温度下贮藏一年后唐山秋瓜超干种子抗氧化酶活性的变化（.doc

目 录 中文摘要……………………………………………………………1 Abstract………………………………………………………………………………2 引言…………………………………………………………………………3 1材料与方法…………………………………………………………………………1.1材料 ……………………………………………………………………………3 讨论 …………………………………………………………………………14 3.1 超干种子的抗氧化系统分析 …………………………………………………14 3.2种子在含水量贮藏问题 ………………………………………………… 15 致谢 …………………………………………………………………………………16 参考文献 ……………………………………………………………………………17 不同温度下贮藏一年后唐山秋瓜超干种子 抗氧化酶活性的变化 摘要：本文测定刚经过超干处理不同含水量的黄瓜种子的丙二醛含量、过氧化氢酶和超氧化物歧化酶活性，愈创木酚过氧化物酶活性，和在不同贮藏温度下贮藏一年后再次测定不同含水量的黄瓜种子的丙二醛含量、过氧化氢酶和超氧化物歧化酶活性，愈创木酚过氧化物酶活性，超氧阴离子自由基活性以及去自由基的消除速率等，来比较不同贮藏温度下，以及不同含水量下，酶系统活性的变化，来研究超干贮藏。结果显示:黄瓜种子含水量降低至不低于2%时，对种子发芽率无不利影响，对种子活力指数也无显著影响。种子过氧化氢酶和超氧物歧化醉等酶系统保持完好，有所下降，但不是特别明显，丙二醛含量有些许升高，也无显著变化。研究认为超干处理唐山秋瓜贮藏一年后酶活性显示黄瓜种子唐山秋瓜适宜超干贮藏，且在含水量2%时，酶活性相对较高，适合贮藏。 关键词：黄瓜种子；超干贮藏；生理生化特性；最适贮藏含水量： Change of antioxidant enzymes activity of cumcumber(cv. tangshan qiugua ) ultra-dry seeds stored at different temperature after a year Student majoring in Seed science and engineering zhufeng Supervisor Yan Min Abstract: This paper deal with just after ultra- dry processing the cucumber seed different moisture to test activity of MDA ，CAT，SOD，POD ，and Superoxide radical activity and to free radical eliminate rate, etc. And in different storage temperature storage a year later test again . To compare different storage temperature, and different water content, the activity of enzyme system changes, to study ultra- dry storage. The results show: Cucumber seed moisture content is reduced to no less than 2% of seed germination rate, no adverse effect on seed vigor index, nor a significant impact CAT,SOD Of Cucumber seed enzyme system remain intact ,just a little drcease.Content of MDA also no significant change in,just a little rise.Studies suggest that ultra-dry processing tangshan autumn melon storage a year later enzyme activity show cucumber seed tangshan autumn melon, and for ultra- dry storage in moisture content 2% when, enzyme activity is relative taller, suitable for storage. Key words:；Cucumber