基因组定向编辑技术——CRISPR Cas9的研究进展.pdfVIP

Hans Journal of Agricultural Sciences 农业科学, 2014, 4, 142-150 Published Online December 2014 in Hans. /journal/hjas /10.12677/hjas.2014.46022 Advances in Genome Directional Editing Technologies of CRISPR/Cas9 Yao Yao, Xueyan Qian, Dongquan Guo* Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun * Email: xzgdq@126.com th th th Received: Nov. 18 , 2014; revised: Nov. 25 , 2014; accepted: Dec. 8 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea. The system is able to degrade the invading virus or phage DNA. Type Ⅱof CRISPR/Cas system has become the most popular method due to its simplicity. This paper summarizes the basic structure, principle, technical characteristics and the progress of CRISPR/Cas, as well as the application prospect of the technology. Keywords CRISPR/Cas System, Cas9 Protein, Targeted Genome Modification 基因组定向编辑技术——CRISPR/Cas9 的研究进展 * 姚 瑶，钱雪艳，郭东全 吉林省农业科学院农业生物技术研究所，长春 * Email: xzgdq@126.com 收稿日期：2014年11月18 日；修回日期：2014年11月25 日；录用日期：2014年12月8 日 *通讯作者。 142 基因组定向编辑技术——CRISPR/Cas9 的研究进展 摘 要 CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats/CRISPR-associated)是 广泛存在于细菌及古生菌中的，由细菌体长期进化而形成的，能够降解入侵