黄芩—柴胡“药对”配伍的药学与药代动力学的分析研究.pdfVIP

Pharmaceutical and Pharmacokinetic Research on Radix Scutellare and Radix Bupleuri in compatibility Speciality: Traditional Chinese Medicine Tutor: Feng-qin Liu Author: Ming-hua Liu Abstract Objective: To analyze effective substance of Radix Scutellare and Radix Bupleuri in compatibility enhancing and changing the treatment that was proved to enhance drug effect by tcm clinical practice and pharmacological effect, in order to provide theoretical basis of modern medicine science for reasonable combination of tcm doctors prescribing medicine. Methods: Through consulting the related literature and learning about the research survey of Scutellaria and Bupleurum to lay a foundation for the experiments; high performance liquid chromatographic was used to determine the contents of baicalin and saikosaponin in sample solution；make reference to the clinical use of the drug, take extracting solvent, dosage, soaking time and extracting times by the four factors and three 4 levels orthogonal test L9(3 ),and choose the optimum extraction process of tested plants. And baicalin contents in the plasma were measured by high performance liquid chromatography; the parameters of pharmacokinetics were calculated by DAS2.0 software. Results: The influence of extraction solvent on extraction ratio of medical components from Radix Scutellare and Radix Bupleuri in compatibility was studied in pharmacy experiment, and with baicalin as indicators, the alcohol extraction process of the drug pair was better than the extraction. The experimental results showed that combined decoction raised the solubilization capacity obviously, and the rationality and validity of the Chin